Transplastomic Plants in Tobacco,Plastid Genome,Transplastomic,Subsequently,Spectinomycin

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Transplastomic Plants and Chloroplast Engineering Transplastomic Plants and Chloroplast Engineering Introduction Engineering of Chrloroplast Genome in Chlamydomonas Transformation of Chloroplast (Cp) Genome in Higher Plants Advantages of Chloroplast Transformation Difficulties in Producing Transplastomic Plants Methods of Tranformation of Cp Genome Agrobacterium Mediated Transformation of Cp Genome Stable Transformation of Cp Genome Using Particle Gun Method Other Possible Methods for Introduction of DNA into Plastids DNA Uptake by Chloroplasts in vivo

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Transplastomic Plants in Tobacco
Transplastomic tobacco for antibiotic/ herbicide resistance. During 1990-2002, a number of efforts were made, where transplastomic tobacco plants were obtained successfully. Two examples of plastid transformation in tobacco have been described earlier in this chapter, while discussing the methods of plastid transformation. In the first ever effort in 1990, plastid transformation was achieved by bombardment of leaves with particles coated with pZS148 plasmid DNA containing a 3.7 kb plastid DNA fragment encoding the l6S rRNA that carried a gene for resistance against spectinomycin (intended to be transferred to the plastid genome), physically linked to the selectable marker gene for streptomycin resistance.

Transplastomic lines were selected by spectinomycin resistance, which resulted by a multistep process involving DNA recombination, copy correction and. sorting out plastid DNA copies. Transgenic plastid trait (antibiotic resistance) was transmitted faithfully to the progeny. Subsequently transfer of genes (including genes for herbicide resistance) that were not linked physically to the selectable marker was also achieved successfully in several studies conducted during 1995-2002.

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Microinjection of DNA Chloroplasts in Vivo DNA Uptake by Chloroplasts in Vitro Removal of Antibiotic Resistance Gene from Transplastomic Plants Transplastomic Plants in Tobacco Transplastomic Tobacco for Human Therapecutics Transplastomic Tobacco with three Genes for Insect Resistance Transplastomic Plants Transplastomic in Arabidopsis Transplastomic in Rice Transplastomic in Potato Transplastomic in Tomato Targeting of Foreign Proteins into Chloroplasts Targeting of Foreign Proteins into Mitochondria