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Home >> Plant Biotechnology and Genomics >> Transplastomic Plants and Chloroplast Engineering >>Transplastomics in Tomato

Transplastomics in Tomato
In the year 2001, success was also reported for the production of transplastomic tomato plants utilizing a gene for aminoglycoside-3' -adenyl transferase (AAD) that confers spectinomycin resistance. The transfer of gene was achieved through biolistic bombardment of leaves. The level of the protein encoded by the transferred gene accumulated up to -0.5% of the total soluble cellular protein (TSP).
Such transfer of gene and its expression in the chromoplasts of the fruit like tomato or roots like carrot will be important since such systems can be utilized for the production of oral and edible vaccines.

In tomato transplastomic plants, the transgene in the chromoplast in tomato fruits expressed the transgene to <50% of the expression level achieved in- leaf chloroplast. This system, therefore, paves the way not only for the transfer of genes for agronomically important traits in tomato chloroplasts, but also for efficient production of edible vaccines, pharmaceuticals and antibo dies in fruits of a variety of crop plants.

 

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