PAC-ELISA, Antisera, DAS-ELISA, Rhizobium Populations, Serological Diagnosis, Antigens, Morphology, Aflatoxins, Gibberellins

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Tissue Culture, Micropropagation and Somaclonal Variation Tissue Culture, Micropropagation and Somaclonal Variation Introduction Cell and Organ Differentiation Cytodifferentiation Organgenic Differentiation Shoot bud Differentiation Somatic Embryogenesis Clonal Propagation or Micropropagation Bioreactors for Micropropagation and for bioactive Compounds List of Plant Materials,Which can be Propagated by Tissue Culture Synthetic Seeds for Micropropagation Production of Virus free Plants ELISA for Detection of Viruses Fungi Bacteria, MLOs, Mycotoxins and Hormones DAC-ELISA PAC-ELISA Maintenance of Male Sterile Parents for Hybrids through Micropropagation Micropropagation of Hybrid Plants Somaclonal Variation

Home >> Plant Biotechnology and Genomics >>Tissue Culture, Micropropagation and Somaclonal Variation >>PAC-ELISA

PAC-ELISA
This protein-A coating (PAC) technique involves five steps: (i) the wells are coated with protein-A (1 ng ml^-1), (ii) high dilution of unprocessed specific antisera were added, (iii) test samples were added, (iv) antisera (cross adsorbed with healthy plant components, to minimize non-specific reaction) were added (v) finally the enzyme substrate is added, for colour reaction.

PAC-ELISA

Different Steps Involved in ELISA Test for Detection of Virus Infection (c) PAC-ELISA

The above PAC-ELISA procedure has also been utilized for detection of mycoplasma-like organisms (MLOs) in crude extracts of leaflets and stem of groundnut plants showing witches broom symptoms. DAS-ELISA method has been used for quantitative estimation of bacterial populations. Rhizobium populations were estimated in many soil samples, when the population was not below 10³ cells g^-1 soil. ELISA was also used for the detection of aflatoxins in groundnut seed samples.

ELISA is currently by far the most widely used test in serological diagnosis. It permits detection of antigens of widely varying size and morphology. At ICRISAT, near Hyderabad also, ELISA was successfully utilized for study of viruses, MLOs, Rhizobium and aflatoxins. It can also be used for the detection of mycorrhizal fungi, and gibberellins through the use of monoclonal antibodies (mAbs). At ICRISAT the use of the enzyme penicillase (instead of commonly used costly enzymes like phosphatase, horse radish peroxidase and urease) was standardized (penicillase is cheap and is available in India, while other enzymes need to be imported).

	Home
List of Some Crop Species where Desirable and Heritable Somaclonal Variation Overcoming Crossing Barriers Using Culture Technques Pre-Fertilization Crossing Barriers on Culture Techniques Post-Fertilization Crossing Barriers Embryo Rescue Embryo Culture Ovule Culture Ovary Culture Other Uses of Tissue Culture Endosperm Culture Nucellus Culture Germplasm Storage Examples of Storage of Plant Tissue Culture Status of Tissue Culture Technology in India Micropropagation Research in India Status of Tissue-Culture Technology in India-Semi Commercial Status of Tissue-Culture Technology in India Commercial Scale Production Somaclonal and Gametoclonal Work in India Secondary Metabolities Research in India