Ovule Culture
For a variety of difficult interspecific/Intergeneric crosses involving members of the families Malvaceae, Fabaceae, Cruciferae, Solanaceae, etc. ovules after fertilization have been successfully cultured to obtain mature embryos/seeds. Depending upon when the embryo aborts, the ovules have to be excised any time from soon after fertilization to almost developed fruits, which may sometimes be lost due to premature abscission. However, ovule culture is mainly tried only in those cases, where embryo aborts very early, and embryo culture is not possible due to difficulty of its excision at a very early stage. In some cases, the medium may need to be supplemented with some fruit/vegetable juice to accelerate initial growth.