Heat Shock Genes, Thermal Stress Induces, Vital Genes, Regulating their expression, Highly Conserved, Drosophila, Shock Was Comparable

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Study of Regulatory DNA Sequences Study of Regulatory DNA Sequences - Introduction Transgenes that are Inducible by a Stimulus or Exhibit Tissue Specific Expression In Transgenic Plants Regulatory Sequences of Induced Genes

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Heat shock genes

Thermal stress induces the transcription of a small set of heat shock genes in diverse organisms, and also reduces the expression of many other vital genes. The upstream region of heat shock genes regulating their expression is highly conserved.

Thus, when a gene construct, carrying NPT II reporter gene, fused with upstream region of heat shock gene hsp70 from Drosophila, was introduced into tobacco, induction of NPT II expression due to heat shock was comparable to that of endogenous plant heat shock genes (inducible in all organs except pollen). Similarly, from maize a homologue of above gene hsp70 with 1.1 kilobases of upstream sequence, when introduced in petunia exhibited heat inducibility.

A. heat shock gene hsp6871 from soybean was also introduced In tobacco and the deletion mutants allowed identification of a 36 bp element (heat shock response element or HSRE) responsible to confer heat inducibility in all organs independent of the presence of light.

Interestingly, when this 36 bp sequence was placed upstream from rbcS promotor, a light dependent and organ specific heat shock response was observed (gene expressed due to heat shock only in the presence of light and only in leaves).

This suggested that a negative control element of rbcS did not allow expression in roots and in the dark even in leaves.

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