Gene for chlorophyll a/b binding protein (Cab)

Examples of the transfer of Cab gene from pea, wheat and Nicotiana plumbaginifolia are listed in Table 34.1. In this case, it was shown that a 247 bp sequence for pea CabAB80 (-347 to -100) and a 268 bp sequence (-357 to -89) for wheat Cab-l, when separately fused with heterologous TAT A sequence fused to neomycin phosphotransferase (NPT II) coding sequence, conferred on this coding sequence a light responsive expression.

This regulatory sequence when present caused induction of expression of NPT II by red light and the expression could be reversed by a flash of far-red light as observed in phytochrome mediated expression. In another experiment, negative elements suppressing expression were also identified, when the Cab-E of Nicotiana plumbaginifolia was studied in a similar manner in transgenic plants.