Alcohol Dehydrogenase Gene ADH, Anaerobic Conditions, Oxygen Deprivation, Activity under Aerobic Condition, Upstream Regulatory, Gene Expression

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Study of Regulatory DNA Sequences Study of Regulatory DNA Sequences - Introduction Transgenes that are Inducible by a Stimulus or Exhibit Tissue Specific Expression In Transgenic Plants Regulatory Sequences of Induced Genes

Home >> Plant Biotechnology and Genomics >> Study of Regulatory DNA Sequences >> Alcohol Dehydrogenase Gene ADH

Alcohol dehydrogenase gene (ADH)

Alcohol dehydrogenase gene expresses under anaerobic conditions leading to oxygen deprivation. Using transgenic plants, it could be shown that regulatory sequences upstream to ADH gene from maize lead to suppression (down regulation) of gene activity under aerobic conditions, this activity being normal and uninducible under anaerobic conditions.

The inability of the ADH upstream regulatory sequences to induce (these sequences only suppress) gene expression became evident when these sequences failed to induce chloramphenicol acetyltransferase (CAT) expression under aerobic as well as anaerobic conditions in transgenic tobacco plants.

Other enhancer sequences (from octopine synthase or CaMV35S gene), when transferred at a position upstream of ADH gene, led to induction under anaerobic conditions.

Therefore, with normal conditions, ADH activity is inhibited under aerobic conditions due to negative control, and expression is promoted under anaerobic conditions simply due to the absence of a negative control rather than due to the presence of positive induction.

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