Interspecific Hybrids Produced Through Protoplast Fusion, Brassica Oleracea, Campestris, Tabacum, Alata, Glauca,Glutinosa, Knightiana, Mesophila

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Protoplast Culture Regeneration and Somatic Hybridization Protoplast Culture Regeneration and Somatic Hybridization - Introduction Isolation of Proplasts Mechanical Method Enzymatic Method Direct One Step Method Sequential Two Step Method Purification of Isolated Protoplasts Sedimentation and Washing Flotation Viability and Plating Density of Protoplasts Testing Protoplast Viability Minimum Plating Density MPD and Culturing Individual Protaplasts Protaplast Culture and Regeneration of Plants Isolation of Subprotoplasts Protoplast Fusion and Somatic Hybridization Techniques of Protoplast Fusion

Home >> Plant Biotechnology and Genomics >> Protoplast Culture Regeneration and Somatic Hybridization >> Interspecific Hybrids Produced Through Protoplast Fusion

Interspecific hybrids produced through protoplast fusion

Parent species and their chromosome numbers	Chromosome number of hybrid
Brassica oleracea (2n = 18) + B. Campestris (2n = 20) B. napus (2n = 38) + B. oleracea (2a = 18) B. napus (2a = 38) + B. nigra (2n = 16) B. napus (2n = 38) + B. carinata (2n = 34) B. napus (2n = 38) + B juncea (2n = 36)	Wide variation
Nicotiana glauca (2n = 24) + N. longsdorfii (2n = 18)	56-64
N. tabacum (2n = 48) + N. alata (2n = 18)	66-71
N. tabacum (2n = 48) + N. glauca (2n = 24)	72
N. tabacum (2n = 48) + N. glutinosa (2n = 24)	50-88
N. tabacum (2n = 48) + N. Knightiana (2n = 24)	44-137
N. tabacum (2n = 48) + N. mesophile (2n = 48)	96
N. tabacum (2n = 48) + N. octophora (2n = 24)	48
N. tabacum (2n = 48) + N. rustica (2n = 48)	60-91

N. tabacum (2n = 48) + N. stocktonii (2n = 48)	96
N. tabacum (2n = 48) + N. sylvestris (2n = 24)	72
N. tabacum (2n = 48) + N. phumbaginifolia (2n = 20)	-
Petunia parodii (2n = -48) + P. hybrida (2n = 20)	44-48
P. parodii (2n = 14) + P. hybrida (2n = 14)	46
P. parodii (2n = 48) + P. parviflora (2n = 18)	31-40
Solnum tuberosun (2n = 24, 48) + S. chapcoense (2n = 14)	60
S. tuberosum (2n = 24, 48) + S. brevidens (2n = 24)	-
Lycopersicon esculentum (2n = 24) + L. Peruvianum (2n = 14)	72
Daucua carota (2n = 18) + D. capillifolius (2n = 18)	36, 38
Datura innoxia (2n = 24) + D. capillifolius (2n = 24)	46, 48, 72
D. innoxia (2n = 24) + d. sanguinea (2n = 24)	46, 72, 96
D. innoxia (2n = 24) + D. candida (2n = 24)	-

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Induced Fusion NaNo₃-treatment Treatment with Calcium Ions ca⁺⁺ at High pH Polyethylene Glycol PEG Treatment Electrical Fusion Selection of Fused Protaplasts Chromosome Status of Somatic Hybrids Interspecific Hybrids Produced Through Protoplast Fusion Intergeneric Hybrids Produced Through Protaplast Fusion Use of Protoplast Fusion and Somatic Hybrids Somatic Hybrids for Gene Transfer Asymmetry in Somatic Hybrids for Gene Transfer Somatic Hybrids for Cytoplasmic Male Sterility Cytoplasmic Hybrids or Cybrids Genetic Modification Transformation of Protoplasts

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