Chromosome Status of Somatic Hybrids, Successfully Obtained, Amphiploid, Cytologgical Level, Multiple Fusion, Asymmetric Hybrids

	Home
Protoplast Culture Regeneration and Somatic Hybridization Protoplast Culture Regeneration and Somatic Hybridization - Introduction Isolation of Proplasts Mechanical Method Enzymatic Method Direct One Step Method Sequential Two Step Method Purification of Isolated Protoplasts Sedimentation and Washing Flotation Viability and Plating Density of Protoplasts Testing Protoplast Viability Minimum Plating Density MPD and Culturing Individual Protaplasts Protaplast Culture and Regeneration of Plants Isolation of Subprotoplasts Protoplast Fusion and Somatic Hybridization Techniques of Protoplast Fusion

Home >> Plant Biotechnology and Genomics >> Protoplast Culture Regeneration and Somatic Hybridization >> Chromosome Status of Soamtic Hybrids

Chromosome status of somatic hybrids

Various somatic hybrids successfully obtained through protoplast fusion. The chromosome numbers of these hybrids indicate that only few have exact number expected in a amphiploid.

In view of this, selection will also need to be applied at the cytological level, if ture amphiploids need to be obtained.

The variability in chromosome number in hybrids could be due to any one of the following reasons:

(i) Multiple fusions give a higher chromosome number. In PEG-induced and electro-induced fusions between more than two protoplasts.

(ii) Asymmetric hybrids result from fusion of protoplasts isolated from actively dividing tissue of one parent and quiescent tissue of the other parent.

(iii) Unequal rates of DNA replication in two fusing partners may also give asymmetric hybrids.

(iv) Somaclonal variation in cultured cells used for protoplast isolation may also lead to variation in chromosome number.

	Home
Induced Fusion NaNo₃-treatment Treatment with Calcium Ions ca⁺⁺ at High pH Polyethylene Glycol PEG Treatment Electrical Fusion Selection of Fused Protaplasts Chromosome Status of Somatic Hybrids Interspecific Hybrids Produced Through Protoplast Fusion Intergeneric Hybrids Produced Through Protaplast Fusion Use of Protoplast Fusion and Somatic Hybrids Somatic Hybrids for Gene Transfer Asymmetry in Somatic Hybrids for Gene Transfer Somatic Hybrids for Cytoplasmic Male Sterility Cytoplasmic Hybrids or Cybrids Genetic Modification Transformation of Protoplasts