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Home >> Plant Biotechnology and Genomics >> Production and Uses of Haploids >> Mutation Research

Mutation Research
In, we discussed the use of cell culture in induction and selection of mutations. The use of haploids provide with the following additional advantages in mutation reseach : (i) Due to the presence of only one set of chromosomes, even the recessive mutations are immediately expressed in haploids (unlike diploids, where the recessive mutations express only in segregating single plant progenies grown in M2 generation). (ii) Pollens can be plated on solid medium like single cells and therefore much bigger populations can be screened in the laboratory, without initially growing the plants in the field.

In several crops, desirable mutants have been isolated among haploids derived in culture. These desirable attributes included resistance to drugs (e.g. antibiotics), amino acid analogues, pathotoxins, salts, herbicides, chilling, diseases (caused by fungi, bacteria, viruses and nematodes). Such mutants can be used for a variety of purposes leading to the development of suitable cultivars.

 

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