Analytical Synthetic Breeding for Potato,Wenzel,Protoplast Fusion,Dihaploids,Monoploids,Tetraploids,Regeneration,Monoploid,Cell Cultures

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Production and Uses of Haploids Production and Uses of Haploids Introduction Production of Haploids Anther and Microspore Culture Ovule Culture Chromosome Elimination Somatic Reduction and Chromosome Elimination Mechanism of Chromosome Elimination Detection or Identification of Haploids Use of Haploids in Plant Breeding Programmes Use of Haploids in Species with Disomic Inheritance Hybrid Sorting in Haploid Breeding Some high Yielding Crop Varieties through Hybrid Sorting Using Anther Culture

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Analytical Synthetic Breeding for Potato
Another approach described as ‘analytical synthetic breeding scheme’ was proposed by Wenzel et al. (1979). This takes advantage of protoplast fusion and plant regeneration to bring together different genotypes into a completely heterozygous tetraploid potato. In this approach, dihaploids are used again for the production of monoploids, before the tetraploid is resynthesized. The breeder can evaluate and select the genomes present in monoploids. The selected monoploids are then used for resynthesise of tetraploids through protoplast fusion and regeneration as follows: (monoploid1 + monoploid2) + (monoploid3 + monoploid4). The selection of best somatic hybrids may be feasible on the basis of their growth rate in cell cultures.

The above scheme of analytical synthesis can be easily used in potato, because once the resynthesized tetraploids are regenerated, they can be multiplied via tubers for commercial cultivation. In other autotetraploid crops li9ke alfalfa (Medicago sativa) or orchard grass (Dactylis glomerata), where reproduction is by seed, the above scheme will not be successful. However, the, analytical breeding, described earlier can be used. In alfalfa, where 2n gametes function, the two schemes suggested by Mendiburu and Peloquin (1977) for potato can also be used.

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