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Home >> Plant Biotechnology and Genomics >>Whole Genome Sequencing and Functional Genomics >> Genome Sequences of Five Chromosomes

Genome sequences of five chromosomes
The nucleotide sequence of the DNA contained in Arabidopsischromosomes 2 and 4 were published in December. 1999 and that of the remaining three chromosomes (chromosome I, 3 and 5) were completed in December 2000, which marked the completion of the sequencing work for 115.4 million base pairs (Mb) of about 125 Mb of this higher plant. This was the first higher plant and the fourth eukaryotic genome sequenced then, the other three eukaryotic genomes being those of yeast (Saccharomyces cerevisiae),a nematode worm (Coenorhabditis elegans)and the fruitly (Drosophila melanogaster).Among the eukaryotic genomes sequenced by then, the sequence of Arabidopsisgenome was also the one with fewest gaps in the gene rich regions.

For the first time it also provided sequences of the substantial proportion of centromeric DNA of each of the five chromosomes. The number of genes predicted to be carried by the whole genome comprising the five chromosomes, was 25,498 in genomic DNA sequences that were only 115,409,949 base pairs (the whole genome being about 125 x 106 base pairs). Therefore, the number of genes was higher than those predicted for either the fruitfly (19,099) or those for C. elegans(13,601), the only two higher eukaryotes that were completely sequences by the end of the year 2000.

Five Chromosomes of Arabidopsis, showing duplicated regions, which are connected by bands; duplicated segments in reverse orientation are connected by twisted bands

Five Chromosomes of Arabidopsis Showing Duplicated Regions, Which are Connected by Bands; Duplicated Segments in Reverse Orientation are Connected by Twisted Bands

Duplications and gene redundancy
In Arabidopsisgenome, most estimates suggest the presence of as many as about 26,000 genes (double the number present in C. elegans), although the functions of atleast 50% of these genes were still unknown, when the sequence was published in December, 2000. Surprisingly, 70% of the Arabidopsisgenome is duplicated, the extensive duplications involving all the five chromosomes thus reducing the number of unique genes that differ from each other to less than 15,000.

 

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