Transgenic Plants Production of Cyclodextrins, Glucosyl transferase, Potato tubers, Glucose molecules, Oligosaccharides

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Molecular Farming Pharming Molecular Farming Pharming Introduction Transgenic Plants as Production Systems Transgenic Plants for Recombinant Proteins in Plant Root Exudates Transgenic Plants for Value Added Speciality Crops Transgenic Plants Production of Cyclodextrins Transgenic Plants Bacteria which Produce CGTases Transgenic Plants Production of Polyhydroxybutyrate or PHB Transgenic Plants Production of Human Serum Albumin (HSA) Transgenic Plants Production of Starch Transgenic Plants for Edible Vaccines

Home >> Plant Biotechnology and Genomics >>Molecular Farming Pharming >>Transgenic Plants Production of Cyclodextrins

Transgenic Plants for Value Added Speciality Crops
Following are some of the examples of chemicals that have already bee produced using transgenic plants as production systems, although they may not have been. commercially exploited in all cases:

Production of cyclodextrins
Transfer of bacterial gene (from Klebsiella) for cyclodextrin glucosyl-transferase (CGTase) to potato was achieved successfully, leading to the production of a and β cyclodextrins (CDs) in potato tubers. Cyclodextrins are cyclic oligosaccharides containing six, seven or eight glucose molecules in a, β or g CDs respectively. CDs can be obtained from starch by the action of cyclodextrin glucosyl transferase (CGTase) enzyme, and are useful for pharmaceutical delivery systems, flavour and odour enhancement and for removal of undesired compounds (such as caffeine) from foods.

The major use of CDs is due to their ability to form inclusion complexes with a wide variety of organic compounds. Tissue specific expression of CGTase in potato tuber and its targeting to plastids for action on stored starch was achieved through the use of a chimeric gene construct consisting of the following: (i) a patatin promoter (for tuber specific expression of CGTase gene), (ii) a DNA sequence encoding transit peptide of the small subunit (SSU) of ribulose bisphosphate carboxylase, (iii) the CGTase structural gene from Klebsiella and (iv) 3 region of the gene encoding nopaline synthase (nos). More transgenic plants with CGTase gene may be produced in view of the present high cost and world demand of CDs and due to availability of a number of CGTase genes.

Present and anticipated world market for cyclodextrins
(source : Consortium for Electrochemische Industri, GmBH)

Application	Market (tonnes year-1)
Application	Actual in 1980s	Expected for 1995
Pharmaceuticals	50	2000
Food	700	2500
Cosmetics	50	500
Agriculture	10	100
Chemical industry	30	300
(biotransfonnations, separation, catalysis) Other purposes (e.g. diagnostics)	10	200

	Home
Factors Affecting Choice of Transgenic Plants for Vaccines Transgenic Tobacco, Potato and Banana as Edible Vaccines Transgenics as Edible Vaccines for Hepatitis B The Production of Vaccines in Transgenic Plants Alternative to Transgenic Plant for Vaccine Production Transgenic Plants for Antibodies Production of Antibodies in Transgenic Plants Transgenic Plants as Factories for Biopharmaceuticals Production of Biopharmaceuticals in Transgenic Plants Production of Glucocerebrosidase for 'Gaucher's Disease' Production of Hirudin (an Anticoagulant) for 'Thrombosis'