Transgenic Plants for Recombinant Proteins in Plant Root Exudates, Heterologous, Plant Organs, Leaves, Fruits, Roots

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Transgenic plants for recombinant proteins in plant root exudates
Numerous heterologous recombinant proteins of economic value have been produced in transgenic plants. These proteins are often produced and accumulate in plant organs like leaves, fruits, roots, tubers and seeds. Also these proteins are targeted to different sub-cellular compartments, such as cytoplasm, endoplasmic reticulum (ER) or vacuoles. However, extraction and purification of proteins from biochemically complex tissues is a laborious and expensive process, which becomes a major barrier to large scale production of these proteins in transgenic plants.

Even the in vitro systems for manufacture of recombinant proteins can be slow, low yielding and expensive. In view of this, a plant system based on natural secretion from the roots of the intact plants has been suggested and tried. The plant roots have developed a sophisticated mechanism involving secretion of different chemicals into the rhizosphere. In a study, reported in 1999, three heterologous genes (genes for green fluorescent protein or GFP from jellyfish, Aequore victoria, human placental secreted alkaline phosphatase or SEAP and xylanase from bacterium, Clostridium thermocellum) were used for the production of transgenic plants, demonstrating that root secretion (termed rhizosecretion) can be successfully exploited for the production of recombinant proteins.

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Factors Affecting Choice of Transgenic Plants for Vaccines Transgenic Tobacco, Potato and Banana as Edible Vaccines Transgenics as Edible Vaccines for Hepatitis B The Production of Vaccines in Transgenic Plants Alternative to Transgenic Plant for Vaccine Production Transgenic Plants for Antibodies Production of Antibodies in Transgenic Plants Transgenic Plants as Factories for Biopharmaceuticals Production of Biopharmaceuticals in Transgenic Plants Production of Glucocerebrosidase for 'Gaucher's Disease' Production of Hirudin (an Anticoagulant) for 'Thrombosis'