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Home >> Plant Biotechnology and Genomics >> Genetically Modified-GM Crops and Floricultural Plants >> Insect Resistant Plants based on Bt


Insect resistant plants based on Bt

The above toxin genes (bt2) from B. thuringiensis have been isolated and used for Agrobacterium Ti- plasmid mediated transformation of a number of crop plants including tobacco, cotton and tomato plants. The transgenic plants were resistant to Manducta sexta, a pest of tobacco.

Experiments of feeding the leaves of these plants to larvae of M. Sexta, showed 75%­100% mortality of the larvae, while the control plants carrying no transgenes were severely damaged. The presence of the gene bt2 as well as that of the toxin protein synthesized under its control was also demonstrated by appropriate experiments.

When inheritance of insect resistance was studied using crosses with normal control plants, F1 showed resistance and F2 generation exhibited expected segregation.

Since, a native gene often does not express the gene at high level, truncated versions of genes are synthesized with altered codon usage and elimination of certain sequences (codon usage means that for same amino acids, codons that are efficient in prokaryotes but not in eukaryotes are replaced by other degenerate codons known to be efficient in eukaryotes).

As many as 410 Bt related patents have been issued during 1985-96, 52% of them issued in the North America, 30% in Europe and Russian countries and the remaining 18% mainly in Japan.

Further, about only l/3rd of these patents were related to Bt- biopesticides and the remaining 2/3rd were related to genes or Bt-based transgenic crops. The major Bt genes include CryIA(b), CryIA(c) and Crylll(a).

Bt-based insect resistant transgenic crops, the genes employed, and the commercialization status of these crops are listed.

 

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