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Home >> Plant Biotechnology and Genomics >> Genetically Modified-GM Crops and Floricultural Plants >> Defense Against Oxidative Stress Using Superoxide Dismutase Sod Gene


Defense against oxidative stress using superoxide dismutase (SOD) gene


An oxidative stress is frequently experienced by plant systems, because many stress factors induce the production of reactive oxygen causing oxidative damage within the cell. Protection against this damage is achieved through both enzymatic and non-enzymatic components. Key enzymes involved in this protection are superoxide dismutases (SODs), which convert superoxide redicals, into hydrogen peroxide.

Due to their metal co-factors, three classes of SODs are recognized: (i) Cu/Zn SOD (generally found in the cytosol and chloroplasts and designated as Cyt Cu/Zn SOD and ChI Cu/Zn SOD respectively), (ii) Mn SOD (generally found in mitochondria) and (iii) Fe SOD (generally found in chloroplasts).

Peroxidases and catalases operate closely with SODs for anti-oxidant defense mechanism, involving removal of H2O2 produced due to SODs. Inefficient removal of H2O2 may lead to formation of hydroxyl radicals which are most reactive of all and therefore react with DNA, proteins and lipids.

(H2O2+O2 ? Fe3-+O2+ OH). Overproduction of SOD has been shown to provide protection against (i) herbicides like 'paraquat', also called methyl viologen or Mv (a superoxide generating system); (ii) chilling stress and drought stress; and (iii) ozone damage as witnessed in tobacco and other members of Solanaceae.

It was, however, noticed that enhanced SOD activity in the mitochondria had only a minor effect on ozone tolerance, while enhanced SOD activity in chloroplasts had 3 to 4 bold reduction in visible ozone injury. The most important work in this field has been done by M Van Montagu of Belgium.

Another group working on SODs is led by E. Galun from Israel. Transgenic potato and tobacco have been produced utilizing Cyt and ChI Cu/Zn SOD and Mit Mn SOD. The genes for SODs were isolated either from tomato or from Necotianaplumbaginifoliaand were integrated either in the nuclear genome or in the chloroplast /mitochondrial genomes.

 

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