Antisense SAHH Gene for Resistance against a Broad Spectrum of Viruses, Virus Replication, Broad Spectrum of Viruses

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Home >> Plant Biotechnology and Genomics >> Genetically Modified-GM Crops and Floricultural Plants >> Antisense SAHH Gene for Resistance Against a Broad Spectrum, of Viruses

Antisense SAHH gene for resistance against a broad spectrum of viruses

In all the above approaches, a gene was used which conferred resistance against one specific virus. However, if we could inhibit expression of a gene responsible for a non-essential host cell enzyme, required for virus replication, we can get resistance against a broad spectrum of viruses.

In one such effort, the enzyme S-adenosyl homocysteine hydrolase (SAUH) was chosen' for manipulation. SAHH is responsible for several methylation reactions including 5'-cap mRNA methylation, where S-adenosyl- methionine is used as methyl donor.

If SAHH is inhibited, there will be under-methylation at the 5' end of viral mRNA leading to inhibition of viral replication. SAHH inhibitions have actually been used as antiviral agents and were found to suppress plant viruses in leaf disk assays.

In a study reported in 1995, transgenic tobacco plants were produced which expressed antisense RNA against mRNA of a tobacco SAHH gene. This led to a reduction of SAHH level and consequent inhibition of viral replication/infection in the transgenic plants.

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Antisense Sahh Gene for Resistance Against a Broad Spectrum of Viruses Mutant Gene for a Protein Necessary for Cell to Cell Movement of Virus in the Host Resistance Against Bacterial and Fungal Pathogens Broad Spectrum Disease Resistance Some Pathogens for Which Resistance has been Transferred in Some Crop Plants Resistance Against Abiotic Stresses Resistance Against Strees Drought Salt Heat Freezing Defense Against Water Stress Accumulation of Osmoprotectants Like Glycine Betaine Defense Against Oxidative Stress using Superoxide Dismutase sod Gene Transgenic Plants with Chilling Resistance Desaturation of Fatty Acids Improved Crop Productivity Improved Harvest Index Higher Biomass Production using Bacterial Hemoglobin Gene vhb Transgenic Plants for Hybrid Seed Production Improved Nutritional Quality Some Transgenic Plants Produced for Functional Food and Neutraceuticals Transgenic Plants for Oil Production Rapeseed and ther Oil Crops Proportion of Desired Polyunsaturated Fatty Acids Pufas By Products From Oil Seed Crops Improvement of other Oil Seed Crops Altered Fatty Acid Composition in Brassica and Soybean Seed Oil Lysine Rich Transgenic Crops Canola and Soybean Crops Rich in Vitamin a Golden Rice and Golden Mustard Transgenic Crops Fortified with Iron Transgenic Plants Suitable for Transport and Long Shelf Life Transgenic Plants for Floriculture Transgenic Crops in India Transgenic Lines in Advanced Stage of Development or Field Testing in India