Logo
 Home | Sitemap | Contact us | Search | Language
Left Right
Home >> Plant Biotechnology and Genomics >> Gene Transfer Methods in Plants >>Green Fluorescent Protein Gene

Green Fluorescent Protein Gene.
 The gene coding for green fluorescent protein (GFP), was isolated in the year 1992 from the jelly fish Aequorea victoria. This gene when used for production of transgenic plants, allow non-destructive, real time, in vivoassay. It has been introduced in bacteria, nematodes, fruitfly (Drosophila),mice and several plant species. GFP is a 27 kD monomer, that has the unique characteristic of emitting green light when exposed to ultraviolet or UV light (~ 395 nm) or blue light (~490 nm). This gene differs from other reporter genes, since no substrate or enzyme is needed for detection. Therefore, this will prove to be a universal transgenic marker, because it is species independent. Any plant and its progeny expressitracked  in real time, so that the plants could be easily monitored. When GFP gene is under the control of a constitutive promoter, the leaves fluoresce green when exposed to UV or blue light and viewed under low ambient light, giving contrast to the pinkish hue emitted by non-transgenic plants.

The gene for GFP has been mutagenized, resynthesized for higher expression in plants. These modifications involved altered codons without changing the amino acids coded (codon usage for optimum expression in higher plants). This modified GFP gene gives stable and improved expression in higher plants. This also allows testing of many plants in the same field and will permit detection of any chance escape of the gene to other species growing in the vicinity, which can then be monitored or destroyed as needed.

 

Left Right