Laboratory Requirement for Plant Tissue Culture

Laboratory space
For a standard tissue culture laboratory, the following minimum facilities are necessary: (i) a media room, where glassware and Plasticware are washed, stored and used for the preparation of culture media; this room should be equipped with working benches (including a laminar air flow cabinet), a deepfreeze, a refrigerator, weighing balance, a hot plate-cum-magnetic stirrer, a pH meter, a vacuum pump, and an autoclave; (ii) a culture room, where controlled temperature, diurnal illumination, and humidity are maintained and cultures are grown; the room should have racks for placing culture tubes, flasks, etc. and also a shaking machine for growing suspension cultures