Culture Media and Cell Culture Introduction, Plant cells, Embryos, B-vitamins, Plant tissue, Hormones, Botany Department

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Culture Media and Cell Culture Culture Media and Cell Culture Introduction Laboratory Requirements for Plant Tissue Culture Washing and Sterilization for Plant Tissue Culture Culture Media and their Constituents Culture Media Media Constituents on Culture Media Media Selection on Culture Media

Home >> Plant Biotechnology and Genomics >> Culture Media and Cell Culture >>Culture Media and Cell Culture Introduction

Plant Cell and Tissue Culture:

1. Culture Media and Cell Culture
The beginning of plant cell and tissue culture was made as early as 1898, when a German botanist, G. Haberlandt successfully cultured fully differentiated individual plant cells, isolated from different tissues in several plant species. For about thirty five years (i.e. upto 1934), little further progress in cell culture research was made, although culture of embryos, roots and other tissues was successfully achieved. During 1934-1939, due to discovery of the importance of auxins and B-vitamins, the foundation of plant tissue culture was laid down by three scientists (Gautheret, White and Nobercourt), Even though only small pieces of tissues and not individual differentiated cells could be grown in cultures. During the next twenty years (1940-60), a variety of chemicals (hormones, vitamins, etc) were identified for their effect on cell division, growth and differentiation

Thus, by early 1960S, the methods of in vitro culture of plant cells, tissues and organs were reasonably well developed. Research in this area at that time was also initiated at the Botany Department, Delhi University in India. Consequently, media and culture techniques for a variety of plant materials became known and their uses suggested, which are now being extensively utilized due to the recognition of the significance of plant tissue culture in plant biotechnology. During 1980-2000, once again plant tissue culture research became a thrust area of research due to the renewed emphasis it received in all areas of crop improvement programmes. In several publications, including books, and in syllabi prescribed for biotechnology courses of our universities, plant tissue culture along with plant genetic engineering have become synonymous with plant biotechnology. Keeping this in view, this chapter and the next few chapters are being devoted to various aspects of plant tissues culture. This chapter is devoted to culture media and cell culture techniques

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Media Preparation on Culture Media Cell Culture Isolation of Single Cells Suspension Cultures Culture of Single Cells Bergmann's Cell Plating Technique Applications of Cell Culture Production of Secondary Metabolites Plant Species and Secontary Metabolites Obtained from them using Tissue Culture Techniques