Physical maps in barley
For construction of physical maps in barley, translocation breakpoints in each of the seven chromosomes involving 120 translocations were used as physical landmarks. Since each chromosome was involved in several translocations, it could be divided in sub-regions on the basis of breakpoints, in a manner analogous to that used in the deletion stocks of bread wheat. STS primers from >300 genetically mapped RFLP markers were prepared, and were used with microdissected chromosomes carrying translocated segments as templates for PCR reactions.


