Physical Map of Maize, Enzymes, Genome, Fingerprint, Clones, Maize genome, BAC libraries, IBM Population, Assign markers

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Construction of Molecular Maps and Synteny (Collinearity) Construction of Molecular Maps and Synteny (Collinearity) Introduction Preparation of Genetic Maps Mapping Populations and near Isogenic Lines (NILs) Different Molecular Marker Systems Polymorphic Markers and Genotyping the Maping Population Preparation of Map Using the Software Mapmaker Molecular Genetic Maps (Low/Moderate Density) Details of Molecular Maps in Some Crops Maps for Cereals Millets and Sugarcane Maps in Legumes ( Soybean, Pea, Common, Bean,Alfalfa,Chickpea,Lentil) Maps in Brassicsa Maps in Cotton Maps for Sunflower Maps for Solanaceous Crops Maps for Forest Trees and Fruit Trees Molecular Genetics Maps (High Density) Integrated Genetic Maps Resolution Gap Differs in Maps with Genomes of Different Sizes Uses of Molecular Genetic Maps Mapping of Major Genes of Economic Value Using Moecular Markers Use of Near Isogenic Lines (NILs) for Gene Tagging Some Examples of Initial RELP Linked Mapping of Major Genes of Economic Value in Some Crops Identification and Mapping of Quantitative Trait Loci (QTLs) using Molecular Markers Simple Interval Mapping (SIM) Marker Aided Selection (MAS) in Plant Breeding Identification of Breeding Lines and Varieties using Markers Characterization of Genetic Resources and Estimation of Genetic Diversity using Markers

Home >> Plant Biotechnology and Genomics >>Construction of Molecular Maps and Synteny (Collinearity) >>Physical Map of Maize

Your Ad Here	Physical map of maize Using inbred line B73, three BAC libraries (using three enzymes, HindIII, EcoRI and MhoI)were prepared. Together the three libraries provided 27 fold genome coverage of the genome. The BAC clones of these libraries are being screened using markers earlier mapped using IBM population. The BAC clones are fingerprinted by digesting BAC clones with HindIII,and fractionating the fragments on agarose gel. The gel images are digitized with IMAGE software (Sanger Centre, UK). Contigs were assembled using Fingerprint Contig (FPC) software. In the first instance, 83,000 of the 450,000 BAC fingerprints were assembled into 13,000 contigs and 9,300 singletons (singletons are clones which have no overlap with any other BAC clone).
A variety of markers including 90 RFLPs, -1000 ESTs, a large number of AFLPs and MITES from the maize genome are being used to screen BAC libraries. A number of cDNA clones from sorghum were also used to assign markers to individual BACs. It is also proposed to develop at least 2000 SNPs from ESTs. SNPs will also be developed through direct sequencing of BACs and BAC ends. The resulting information will provide important anchor points for assembling individual BACs into contigs.
MaizeDB can also be accessed at http://www.agron.missouri.eduJ/, where maps, probes, primers, screening images and lab protocols are available. It also allows users to compare sequences of interest to all public maize sequences. For more information maize DB also provides database links including the following: (i) CUG 1 links to BAC contigs (ii) GenBank and ZmDB (Zea maysdatabase at Iowa State University) links for sequence and clone information.

	Home
Study of Phylogenetic Relationships using Molecular Markers Identification of Somatic Hybrids Molecular Cytogenetic Maps using Cytogenetic Stocks Molecular Physical Maps Genome-wide Physical Maps using Large Insert YACs,BACs and BIBACs Intergrated Physical Map of Arabidopsis Thaliana Genomic-wide Physical Map in Indica Rice Physical Map of Maize Physical Maps in Barley Physical Maps Using in Situ Hybridization (ISH) Molecular Maps in Yeast and Other Fungi Physical Sizes of Chromosomes that were Separated by PFGE,in Budding Yeast and Aspergillus Nidutans Comparative Genomics and Collinearity/Synteny in Maps Microcollinearity in DNA Sequences of Small Genomic Regions Large Genomic Fragements Sequenced in Some Cereal Species Molecular Transcripr (EST) Maps Genomics for Evolutionary Studies

Language >> English | Français | Español | Deutsch | Italiano | Portugues | Japanese | Korean | Sim-Chinese