Molecular Cytogenetic Maps using Cytogenetic Stocks, Morphological, Chromosome marker, Tetrasomic lines, Wheat, Cotton

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Construction of Molecular Maps and Synteny (Collinearity) Construction of Molecular Maps and Synteny (Collinearity) Introduction Preparation of Genetic Maps Mapping Populations and near Isogenic Lines (NILs) Different Molecular Marker Systems Polymorphic Markers and Genotyping the Maping Population Preparation of Map Using the Software Mapmaker Molecular Genetic Maps (Low/Moderate Density) Details of Molecular Maps in Some Crops Maps for Cereals Millets and Sugarcane Maps in Legumes ( Soybean, Pea, Common, Bean,Alfalfa,Chickpea,Lentil) Maps in Brassicsa Maps in Cotton Maps for Sunflower Maps for Solanaceous Crops Maps for Forest Trees and Fruit Trees Molecular Genetics Maps (High Density) Integrated Genetic Maps Resolution Gap Differs in Maps with Genomes of Different Sizes Uses of Molecular Genetic Maps Mapping of Major Genes of Economic Value Using Moecular Markers Use of Near Isogenic Lines (NILs) for Gene Tagging Some Examples of Initial RELP Linked Mapping of Major Genes of Economic Value in Some Crops Identification and Mapping of Quantitative Trait Loci (QTLs) using Molecular Markers Simple Interval Mapping (SIM) Marker Aided Selection (MAS) in Plant Breeding Identification of Breeding Lines and Varieties using Markers Characterization of Genetic Resources and Estimation of Genetic Diversity using Markers

Home >> Plant Biotechnology and Genomics >>Construction of Molecular Maps and Synteny (Collinearity) >>Molecular Cytogenetic Maps using Cytogenetic Stocks

Your Ad Here	Molecular Cytogenetic Maps Using Cytogenetic Stocks Through a study of linkage of molecular marker loci with morphological or biochemical (isozymes) markers having known chromosome location, it was possible to locate molecular marker loci not only on specific chromosomes, but also on specific regions of these chromosomes. For this purpose, nullisomictetrasomic lines (in wheat), monosomics (in cotton, maize), trisomics (in barley and rice) and various available translocations (in cotton) or deletion stocks (in wheat), were utilized. This resulted in the preparation of molecular chromosome maps for all major crops.
The above technique also allowed mapping of duplicate loci, when more than one fragments homologous to a probe were detected on Southern blots or more than one PCR products obtained using same SSR primer pair. While in maize, 28.6% sequences detected duplicate loci, in tomato these duplicate loci were less that 2% of the loci tested. This gave new evidence about the presence of frequent duplicate chromosome segments in maize rather than in tomato. More recently, duplicate loci have also been detected using whole genome sequencing, as done in Arabidopsis.
Molecular chromosome maps are also being prepared for wheat, rye, barley, rice, etc. and the availability of aneuploids has greatly facilitated this work. For instance in wheat the use of nullisomic-tetrasomic lines facilitated the location of marker loci on specific chromosomes and the use of telocentrics allowed their location on specific arms. This has thus allowed in wheat the identification of molecular markers for each of the chromosomes arms belonging to 21 different chromosomes. In this connection, under the name 'International Triticeae Mapping Initiative (ITMI)', international collaboration was initiated to prepare maps for the tribe Triticeae and each homoeologous group was assigned to one research group. Twelve such workshops to assess the progress made and to plan future work were organised during 1990-2002. Rapid progress in preparation of molecular maps of different species of the Triticeae was made through these efforts.
Alien addition lines in wheat have also been utilized for assigning genes to specific chromosomes of the alien species involved. For instance wheat-barley addition lines have been successfully utilized for assigning genes on specific barley chromosomes and wheat-rye addition lines were used for a similar purpose for assigning genes on specific rye chromosomes. Oat-maize addition lines were similarly used for assigning markers to individual maize chromosomes. Genome specific probes (isolated from DNA of Aegilops squarrosa)have also been isolated to identify the D genome chromosomes in wheat and several other polyploid species carrying the D genome e.g. Ae.cylindrica,CCDD; Ae. crassa,DDMM; Ae. ventricosa, DDUU.

	Home
Study of Phylogenetic Relationships using Molecular Markers Identification of Somatic Hybrids Molecular Cytogenetic Maps using Cytogenetic Stocks Molecular Physical Maps Genome-wide Physical Maps using Large Insert YACs,BACs and BIBACs Intergrated Physical Map of Arabidopsis Thaliana Genomic-wide Physical Map in Indica Rice Physical Map of Maize Physical Maps in Barley Physical Maps Using in Situ Hybridization (ISH) Molecular Maps in Yeast and Other Fungi Physical Sizes of Chromosomes that were Separated by PFGE,in Budding Yeast and Aspergillus Nidutans Comparative Genomics and Collinearity/Synteny in Maps Microcollinearity in DNA Sequences of Small Genomic Regions Large Genomic Fragements Sequenced in Some Cereal Species Molecular Transcripr (EST) Maps Genomics for Evolutionary Studies

Language >> English | Français | Español | Deutsch | Italiano | Portugues | Japanese | Korean | Sim-Chinese