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Home >> Plant Biotechnology and Genomics >>Construction of Molecular Maps and Synteny (Collinearity) >>Molecular Transcripr (EST) Maps

Molecular Transcript (EST) Maps
With the emphasis on functional genomics research, and with accumulation of EST data for number of plant genome, efforts were also underway at the beginning of the present century to prepare transcript maps in crop plants. These maps could be genetic, cytogenetic or physical in nature. A composite map with 272 loci (spread over 1573 cM) of the expressed sequences and phenotypic traits in sunflower was published in 1999. The construction of this map involved anonymous cDNA markers.

A rice transcript map was published in 2002 and barley transcript map will be published in 2003. The rice transcript map contained as many as 6591 EST sites, occupying 80.8% of the genome. I was shown that 40% of ESTs occupied only 21% of the genome, suggesting that genes are not randomly distributed and that there are gene rich and gene-poor regions in the genomes of crop plants. A transcript map in barley is being constructed at IPK, Gatersleben (Germany) and should be published sometime in 2003. Ten different laboratories in USA are also busy in preparing a transcript map in wheat involving 10,000 unique ESTs.

 

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