Intergrated Physical Map of Arabidopsis Thaliana, Heterochromatic, Centromeric regions, Arabidospis, Fingerprinting

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Construction of Molecular Maps and Synteny (Collinearity) Construction of Molecular Maps and Synteny (Collinearity) Introduction Preparation of Genetic Maps Mapping Populations and near Isogenic Lines (NILs) Different Molecular Marker Systems Polymorphic Markers and Genotyping the Maping Population Preparation of Map Using the Software Mapmaker Molecular Genetic Maps (Low/Moderate Density) Details of Molecular Maps in Some Crops Maps for Cereals Millets and Sugarcane Maps in Legumes ( Soybean, Pea, Common, Bean,Alfalfa,Chickpea,Lentil) Maps in Brassicsa Maps in Cotton Maps for Sunflower Maps for Solanaceous Crops Maps for Forest Trees and Fruit Trees Molecular Genetics Maps (High Density) Integrated Genetic Maps Resolution Gap Differs in Maps with Genomes of Different Sizes Uses of Molecular Genetic Maps Mapping of Major Genes of Economic Value Using Moecular Markers Use of Near Isogenic Lines (NILs) for Gene Tagging Some Examples of Initial RELP Linked Mapping of Major Genes of Economic Value in Some Crops Identification and Mapping of Quantitative Trait Loci (QTLs) using Molecular Markers Simple Interval Mapping (SIM) Marker Aided Selection (MAS) in Plant Breeding Identification of Breeding Lines and Varieties using Markers Characterization of Genetic Resources and Estimation of Genetic Diversity using Markers

Home >> Plant Biotechnology and Genomics >>Construction of Molecular Maps and Synteny (Collinearity) >>Intergrated Physical Map of Arabidopsis Thaliana

Your Ad Here	Integrated physical map of Arabidopsis thaliana During 1990-2000, several genome-wide physical maps of Arabidopsis thalianagenome were developed using large-insert YACs or BACs. These maps were also used for working out the whole genome sequences, which were published in December, 2000. The whole genome sequence, however, still had gaps in the heterochromatic and centromeric regions. In order to fill up these gaps, a whole-genome integrated physical map in Arabidopsiswas prepared and published towards the end of the year 2001. For preparing this latest physical map, a new BIBAC library was prepared, which complemented the BAC libraries used earlier for mapping.
As many as 10,752 BAC and BIBAC clones were used for restriction fingerprinting. Based on fingerprints, the BAC/BIBAC clones were assembled into 196 contigs, which included one contig belonging to chloroplast DNA, another contig belonging to mitochondrial DNA and the remaining 194 clones belonging to >126Mb of the nuclear genome. The physical map thus prepared was integrated with the available maps based on BACs and whole genome sequence. Approximately 95% of the new BAC/BIBAC contigs used for integrated physical maps were consistent with contigs of the existing physical and sequence maps, both in clone content and clone order, but 5% of contigs differed.
The accuracy of the above integrated physical map was also checked using DNA markers. The large-insert clones earlier used for mapping were available in BAC vectors, which required subcloning before they could be used in transformation experiments for functional analysis. This problem was largely overcome through the use of a large number of BIBAC clones in the above integrated map, which being binary in nature, could be directly (without subcloning) used in transformation studies for functional analysis
Sequence analysis of Arabidopsisgenome had earlier suggested that it contained 25,498 genes. However, the functions of >50% these genes remained to be characterized experimentally, a goal that is planned to be achieved by 2010 through the Arabidopsis"2010 Project" (consult next chapter for details). This work will be greatly facilitated by the availability of the above integrated whole-genome physical map.

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Study of Phylogenetic Relationships using Molecular Markers Identification of Somatic Hybrids Molecular Cytogenetic Maps using Cytogenetic Stocks Molecular Physical Maps Genome-wide Physical Maps using Large Insert YACs,BACs and BIBACs Intergrated Physical Map of Arabidopsis Thaliana Genomic-wide Physical Map in Indica Rice Physical Map of Maize Physical Maps in Barley Physical Maps Using in Situ Hybridization (ISH) Molecular Maps in Yeast and Other Fungi Physical Sizes of Chromosomes that were Separated by PFGE,in Budding Yeast and Aspergillus Nidutans Comparative Genomics and Collinearity/Synteny in Maps Microcollinearity in DNA Sequences of Small Genomic Regions Large Genomic Fragements Sequenced in Some Cereal Species Molecular Transcripr (EST) Maps Genomics for Evolutionary Studies

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