Identification of Somatic Hybrids, Protoplasts, Green leaf mesophyll, Manual isolation, Heterokaryons, Auxotrophic, Hybrid vigour

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Construction of Molecular Maps and Synteny (Collinearity) Construction of Molecular Maps and Synteny (Collinearity) Introduction Preparation of Genetic Maps Mapping Populations and near Isogenic Lines (NILs) Different Molecular Marker Systems Polymorphic Markers and Genotyping the Maping Population Preparation of Map Using the Software Mapmaker Molecular Genetic Maps (Low/Moderate Density) Details of Molecular Maps in Some Crops Maps for Cereals Millets and Sugarcane Maps in Legumes ( Soybean, Pea, Common, Bean,Alfalfa,Chickpea,Lentil) Maps in Brassicsa Maps in Cotton Maps for Sunflower Maps for Solanaceous Crops Maps for Forest Trees and Fruit Trees Molecular Genetics Maps (High Density) Integrated Genetic Maps Resolution Gap Differs in Maps with Genomes of Different Sizes Uses of Molecular Genetic Maps Mapping of Major Genes of Economic Value Using Moecular Markers Use of Near Isogenic Lines (NILs) for Gene Tagging Some Examples of Initial RELP Linked Mapping of Major Genes of Economic Value in Some Crops Identification and Mapping of Quantitative Trait Loci (QTLs) using Molecular Markers Simple Interval Mapping (SIM) Marker Aided Selection (MAS) in Plant Breeding Identification of Breeding Lines and Varieties using Markers Characterization of Genetic Resources and Estimation of Genetic Diversity using Markers

Home >> Plant Biotechnology and Genomics >>Construction of Molecular Maps and Synteny (Collinearity) >>Identification of Somatic Hybrids

Your Ad Here

Identification of somatic hybrids
One of the problems in production of somatic hybrids, often, is lack of appropriate methods for identification of these hybrids among the developing regenerants Some of the methods traditionally used, include the following: (i) combining light bleached protoplasts with green leaf mesophyll protoplasts, followed by manual isolation of heterokaryons; (ii) genetic complementation of auxotrophic mutants; (iii) fusion of different antibiotic and/or herbicide - resistant lines derived by plant transformation and (iv) use of hybrid vigour, isozymes, proteins, etc. These methods, however, are time consuming and costly.

Molecular markers can be utilized for the same purpose, where a small DNA sample from developing regenerant can be used to determine hybridity. In case of PCR-based markers (RAPDs, SSRs, etc.) primers may be used which give polymorphic PCR products from each of the parents involved in fusion, so that the hybrids can be identified by the presence of molecular profiles, which represented a combination of the profiles of the two parents. This has been achieved successfully in several cases including potato and the approach will certainly be utilized in future for identification of somatic hybrids in a variety of plant materials.

	Home
Study of Phylogenetic Relationships using Molecular Markers Identification of Somatic Hybrids Molecular Cytogenetic Maps using Cytogenetic Stocks Molecular Physical Maps Genome-wide Physical Maps using Large Insert YACs,BACs and BIBACs Intergrated Physical Map of Arabidopsis Thaliana Genomic-wide Physical Map in Indica Rice Physical Map of Maize Physical Maps in Barley Physical Maps Using in Situ Hybridization (ISH) Molecular Maps in Yeast and Other Fungi Physical Sizes of Chromosomes that were Separated by PFGE,in Budding Yeast and Aspergillus Nidutans Comparative Genomics and Collinearity/Synteny in Maps Microcollinearity in DNA Sequences of Small Genomic Regions Large Genomic Fragements Sequenced in Some Cereal Species Molecular Transcripr (EST) Maps Genomics for Evolutionary Studies