Genome-wide Physical Maps using Large Insert YACs,BACs and BIBACs, Genomics, Fingerprinting, Whole-genome sequencing

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Construction of Molecular Maps and Synteny (Collinearity) Construction of Molecular Maps and Synteny (Collinearity) Introduction Preparation of Genetic Maps Mapping Populations and near Isogenic Lines (NILs) Different Molecular Marker Systems Polymorphic Markers and Genotyping the Maping Population Preparation of Map Using the Software Mapmaker Molecular Genetic Maps (Low/Moderate Density) Details of Molecular Maps in Some Crops Maps for Cereals Millets and Sugarcane Maps in Legumes ( Soybean, Pea, Common, Bean,Alfalfa,Chickpea,Lentil) Maps in Brassicsa Maps in Cotton Maps for Sunflower Maps for Solanaceous Crops Maps for Forest Trees and Fruit Trees Molecular Genetics Maps (High Density) Integrated Genetic Maps Resolution Gap Differs in Maps with Genomes of Different Sizes Uses of Molecular Genetic Maps Mapping of Major Genes of Economic Value Using Moecular Markers Use of Near Isogenic Lines (NILs) for Gene Tagging Some Examples of Initial RELP Linked Mapping of Major Genes of Economic Value in Some Crops Identification and Mapping of Quantitative Trait Loci (QTLs) using Molecular Markers Simple Interval Mapping (SIM) Marker Aided Selection (MAS) in Plant Breeding Identification of Breeding Lines and Varieties using Markers Characterization of Genetic Resources and Estimation of Genetic Diversity using Markers

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Genome-wide physical maps using large-insert YACs, BACs and BIBACs
In all plants and animals, in order to facilitite genomics research, genome-wide physical maps are now being prepared using large-inserts in YAC, BAC and BIBAC vectors. These maps provide essential platforms for a variety of activities including the following: (i) large scale genome-sequencing, (ii) effective positional cloning, (iii) high throughput physical mapping of ESTs and (iv) target DNA-marker development. Several approaches that are available for the development of these large-insert physical maps include the following: (i) hybridization- based methods using interative hybridizations

(ii) restriction-based fingerprinting methods and (iii) methods for development of integrated maps involving BAC end sequencing, fingerprinting and whole-genome sequencing. Of these three methods, the hybridization method suffers with the problem of the occurrence of repeat sequences, while integrated-map approach, based on sequencing and fingerprinting, is expensive. Therefore, the method of choice during the year 2001 has been the restriction-based fingerprinting. In this restriction fingerprinting approach, the restricted fragments are end labelled and fractionated on agarose or denaturing polyacrylamide gels. During 2001, automatic sequencers were also used for electrophoresis-based fingerprinting, leading to the preparation of large-insert BAC-based physical maps in an indica rice

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Study of Phylogenetic Relationships using Molecular Markers Identification of Somatic Hybrids Molecular Cytogenetic Maps using Cytogenetic Stocks Molecular Physical Maps Genome-wide Physical Maps using Large Insert YACs,BACs and BIBACs Intergrated Physical Map of Arabidopsis Thaliana Genomic-wide Physical Map in Indica Rice Physical Map of Maize Physical Maps in Barley Physical Maps Using in Situ Hybridization (ISH) Molecular Maps in Yeast and Other Fungi Physical Sizes of Chromosomes that were Separated by PFGE,in Budding Yeast and Aspergillus Nidutans Comparative Genomics and Collinearity/Synteny in Maps Microcollinearity in DNA Sequences of Small Genomic Regions Large Genomic Fragements Sequenced in Some Cereal Species Molecular Transcripr (EST) Maps Genomics for Evolutionary Studies