Construction of Molecular Maps and Synteny (Collinearity) Introduction, Polymorphisms, Molecular Biology, Plant Biotechnology

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Construction of Molecular Maps and Synteny (Collinearity) Construction of Molecular Maps and Synteny (Collinearity) Introduction Preparation of Genetic Maps Mapping Populations and near Isogenic Lines (NILs) Different Molecular Marker Systems Polymorphic Markers and Genotyping the Maping Population Preparation of Map Using the Software Mapmaker Molecular Genetic Maps (Low/Moderate Density) Details of Molecular Maps in Some Crops Maps for Cereals Millets and Sugarcane Maps in Legumes ( Soybean, Pea, Common, Bean,Alfalfa,Chickpea,Lentil) Maps in Brassicsa Maps in Cotton Maps for Sunflower Maps for Solanaceous Crops Maps for Forest Trees and Fruit Trees Molecular Genetics Maps (High Density) Integrated Genetic Maps Resolution Gap Differs in Maps with Genomes of Different Sizes Uses of Molecular Genetic Maps Mapping of Major Genes of Economic Value Using Moecular Markers Use of Near Isogenic Lines (NILs) for Gene Tagging Some Examples of Initial RELP Linked Mapping of Major Genes of Economic Value in Some Crops Identification and Mapping of Quantitative Trait Loci (QTLs) using Molecular Markers Simple Interval Mapping (SIM) Marker Aided Selection (MAS) in Plant Breeding Identification of Breeding Lines and Varieties using Markers Characterization of Genetic Resources and Estimation of Genetic Diversity using Markers

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Plant Genomics

1. Construction of Molecular Maps and Synteny (Collinearity)
The detection and exploitation of naturally occurring DNA sequence polymprphisms represent one of the most significant development in molecular biology. Earlier in the techniques of development of molecular markers like RFLP, RAPD, AFLP, SSR, STS (sequence tagged site) and EST (expressed sequence tag) were described. The preparation of the molecular maps using these markers for animal genomes was described in the preparation of similar molecular maps in crop plants has been a priority area of research in plant biotechnology also.

The utility of these maps for crop improvement programmes has been emphasized during 1980s, so that the molecular maps have already been prepared for all major crop species. The techniques of the use of molecular markers have also proved useful for characterization of crop varieties and plant genetic resources through DNA fingerprinting. More recently YAC and BAC physical maps have also been prepared and used for ‘clone to clone’ genome sequencing. These aspects of plant biotechnology will be briefly discussed in this chapter (for details of molecular markers, for methods involved in preparation and use of BAC/YAC maps for whole genome sequencing).

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Study of Phylogenetic Relationships using Molecular Markers Identification of Somatic Hybrids Molecular Cytogenetic Maps using Cytogenetic Stocks Molecular Physical Maps Genome-wide Physical Maps using Large Insert YACs,BACs and BIBACs Intergrated Physical Map of Arabidopsis Thaliana Genomic-wide Physical Map in Indica Rice Physical Map of Maize Physical Maps in Barley Physical Maps Using in Situ Hybridization (ISH) Molecular Maps in Yeast and Other Fungi Physical Sizes of Chromosomes that were Separated by PFGE,in Budding Yeast and Aspergillus Nidutans Comparative Genomics and Collinearity/Synteny in Maps Microcollinearity in DNA Sequences of Small Genomic Regions Large Genomic Fragements Sequenced in Some Cereal Species Molecular Transcripr (EST) Maps Genomics for Evolutionary Studies