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  Home >> Molecular Biology Dictionary >> Kinin and Knock - out experiment


Kinin

A substance promoting cell division. In plant systems, the prefix cyto-has been added (cytokinin) to distinguish it form kinin in animal systems.

Klenow fragment
A product of proteolytic digestion of the DNA polymerase I from E. coli; it has both polymerase and 3-exonuclease activities but not 5-exonuclease activity.

Km
A dissociation constant that characterizes the binding of an enzyme to a substrate. The smaller the vale of Km, the tighter the binding of the enzyme to the substrate. Also called the Michaelis constant.

Knockout
An animal resulting from an embryonic stem cell in which a normal functional gene has been replaced by a non-functional form of the gene. This technique is used extensively in mice-much can be learned about the function of a gene by studying the phenotype of animals that lack peptide product of the gene.

Knock-out experiment
A technique for deleting, mutating or otherwise inactivating a gene in a mouse. This laborious method involves transfecting a crippled gene into cultured embryonic stem cells, searching through the thousands of resulting clones for one in which the crippled gene exactly replaced the normal one (by homologous recombination), and inserting that cell back into a mouse blastocyst.
The resulting mouse will be chimaeric but, if you are lucky (and if you’ve gotten this far, you obviously are), its germ cells will carry the deleted gene. A few rounds of careful breeding can then deleted gene. A few rounds of careful breeding can then produce progeny in which both copies of the gene are inactivated.

 

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