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  Home >> Molecular Biology Dictionary >> Homomultimeter Homoloymer - Hybird seed

Homomultimer
See homopolymer.

Homoplasmy
A cellular condition in which all copies of an organelle are genetically identical. See heteroplasmy.

Hybrid arrested translation
A method used to identify the proteins encoded by a cloned DNA. The cloned DNA is bound to a nitrocellulose filter and a crude preparation of mRNA is hybridized to the filter bound DNA. Only mRNA sequences homologous to the cloned DNA will be retained on the filter. These mRNA molecules can then be removed by high temperature or by using Formamide. The purified mRNA is then placed in an in vitro translation system and the proteins encoded by the message can be analysed by electrophoresis through a polyacrylamide gel. See hybrid arrested translation.

Homopolyer
1. A nucleic acid strand that is composed of one kind of nucleotide, e.g., GGGGGGGG. See polymer.
2. A polymer having a chain structure in which all mer units are of the same type

.Homopolymeric tailing
See tailing.

Hybrid dysgenesis
A syndrome of abnormal germline traits, including mutation, chromosome breakage, and sterility, which results from activity of transposable elements.

Homozygote
(adj: homozygous) (Gr. homos, one and the same + zygon, yoke) An individual that has two copies of the same allele at a particular locus in its two homologous chromosomes. See allele; genotype; heterozygote.

Hybrid arrested translation

A method used to identify the proteins encoded by a cloned DNA sequence. A crude cellular mRNA preparation, composed of many individual types of mRNA, is hybridized with cloned DNA. Only mRNA molecules homologous to the cloned DNA will anneal to it. The rest of the mRNA molecules are put into an in vitro translation system and the protein products are compared with the proteins obtained by  use of the whole mRNA preparation. Cg hybrid released translation.

Hybrid cell
The mononucleate cell which results from the fusion of two different cells, leading to the formation of a synkaryon.

Host strain (bacterial)
The bacterium used to harbor a plasmid. Typical host strains include HB101 (general purpose E. coli strain), DH5a (ditto), JM101 and JM109 (suitable for growing M13 phages), XL1- Blue (general purpose, good for blue/white lacZ screening). Note that the host strain is available in a form with no plasmids (hence you can put one of your own into it), or it may have plasmids present (especially if you put them there). Hundreds, perhaps thousands, of host strains are available.

Hybrid seed
1. Seed produced by crossing genetically dissimilar parents. 2. In plant breeding, used colloquially for seed produced by specific crossed of carefully selected pure lines, such that the F1 crop displays vigour. As the F1 crop is heterozygous, it does not breed true and so new seed must be purchased each season.

Hup+
See hydrogen uptake positive.

Hybrid
(L. hybrida) 1. The offspring of two parents that are genetically different.  A cross between two genetically unlike individuals. 2. A heteroduplex DNA or DNA-RNA molemule


 

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