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  Home >> Molecular Biology Dictionary >> Gene Cloning - Gene Pool

Gene cloning
The process of synthesizing multiple copies of a particular DNA sequence using a bacteria cell or another organism as a host. The gene of interest is inserted into a self replicating DNA molecule (DNA vector, often a plasmid) and the resulting recombinant DNA molecule is amplified in an appropriate host cell. Used in genetic engineering a.k.a. molecular cloning; cloning. See DNA;host.

Gene conservation; genetic resources conservation
The conservation of species, populations, individuals or parts of individuals, by in situ or ex situ methods, to provide a diversity of genetic materials for present and future generations.

Gene library 
Collection of cloned DNA fragments that ideally includes all the genetic information of an organism. If the original source of the DNA is the genomic DNA from an organism, then it is called a genomic library; if the DNA is from cDNA made by enzymatic copying of RNA, then the library includes representative fragments from all genes that were being expressed at the time the RNA was sampled; and would be called a cDNA library. a.k.a. gene bank; library; clone bank; bank; library.

Gene 'linkage
See linkage.

Gene modification
Chemical change to a gene's DNA sequence. See DNA.

Gene conversion
A process, often associated with recombination, during which one allele is replicated at the expense of another, leading to non Mendelian segregation ratios. In whole tetrads, for example, the ratio may be 6:2 or 5:3 instead of the expected 4:4.

Gene frequency
See allele frequency.

Gene imprinting
The differential expression of a single gene according to its parental origin.

Gene insertion
The incorporation of one or more copies of a gene into a chromosome.

Gene interaction
Modification of gene action by a non allelic gene.

Gene expression
The process by which a gene produces RNA and protein and hence exerts its effects on the phenotype of an organism.

Gene flow
The spread of genes from one breeding population to another (usually) related populations by migration, possibly leading to changes in allele frequency.

Gene pool
1. The total genetic information in all the' genes in 'a breeding population at a given time. 2. In PGR: Use is made of the concept of 1°,2" and 3° (primary, secondary and tertiary) gene pools. In general, members of a 10 gene pool are inter fertile; those of the 20 gene pool can cross with the 10 gene pool under special circumstances; with the 3° gene pool, extreme techniques are required to achieve crossing.

 

 

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