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  Home >> Molecular Biology Dictionary >> GC Island - Gene Bank


GC island
A segment of DNA that is rich in G=C base pairs and often precedes a transcribed gene in the genomes of vertebrate organisms.

GDP
Guanosine diphosphate, see Guanosine.

Gel electrophoresis
A method to analyze the size of DNA (or RNA) fragments. In the presence of an electric field, larger fragments of DNA move through a gel slower than smaller ones. If a sample contains fragments at four different discrete sizes, those four size classes will, when subjected to electrophoresis, all migrate in groups, producing four migrating “brands”. Usually, these are visualized by soaking the gel in a dye (Ethidium bromide) which makes the DNA fluoresce under UV light.

Gel Electrophoresis - Voltage
Gel Electrophoresis - Voltage

Gel shift assay
(aka gel mobility shift assay (GMSA), band shift assay (BSA), electrophoretic mobility shift assay (EMSA) A method by which one can determine whether a particular protein preparation contains factors which bind to a particular DNA fragment. When a radiolabeled DNA fragment is run on a gel, it shows a characteristic mobility. If it is first incubated with a cellular extract of proteins (or with purified protein), any protein DNA complexes will migrate slower than the naked DNA a shifted band.

GelriteTM
The brand name of a pseudomonas derived refined polysaccharide used as a gelling agent and agar substitute.

GEM
Genetically engineered micro organism.

Gene addition
The addition of a functional copy of a gene to the genome of an organism.

Gene Amplification
The selective production of multiple gene copies without a proportional increases in others.

Gene
1. (Gr. gen, race, offspring). Conceptually, the unit of heredity transmitted from generation to generation during asexual or asexual reproduction. More generally, the term is used in relation to the transmission and inheritance of particular identifiable traits. Since the molecular revolution, it is now known that a gene is a segment of nucleic acid that encodes peptide or RNA.

2. See polygenes; dominant gene; fusion gene; gene amplification; gene expression; gene flow; gene pool; gene splicing; gene translocation; recessive gene; regulatory gene.

3. A unit of DNA which performs one function. Usually, this is equated with the production of one RNA or one protein. A gene contains coding regions, introns, untranslated regions and control regions.

Gene bank
1. The physical location where collections of genetic material in the form of seeds, tissues or reproductive cells of plants or animals are stored. 2. Field gene bank: A facility established for the ex situ storage and maintenance, using horticultural techniques, of individual plants. Used for species whose seeds are recalcitrant (Q.v), or for clonally propagated species of agricultural importance, such as apple varieties). 3. A collection of cloned DNA fragments from a single genome. Ideally the bank should contain cloned representatives of all the DNA sequences in the genome. 4. A population of micro organisms, each of which carries a DNA molecule that was inserted into a cloning vector. Ideally, all of the cloned DNA molecules together represent the entire genome of another organism. Also called gene library, clone bank, bank, library. This term is sometimes also used to denote all of the vector molecules, each carrying a piece of the chromosomal DNA of an organism, prior to the insertion of these molecules into a population of host  cells.

 

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