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  Home >> Molecular Biology Dictionary >> DNA Probe - DNA Domiance

DNA probe
A labelled (tagged) segment of DNA that is able, after a DNA hybridization reaction, to detect a specific DNA sequence in a mixture of sequences. If the tagged sequence is complementary to anyone in the mixture, the two sequences will form a double helix. This will be identified thanks to its label (either by radioactivity or fluorescence)

DNA repair
A variety of mechanisms that repair errors that occur during DNA replication.

DNA repair enzymes
Enzymes that catalyse the repair of DNA.

DNA replication
The process whereby DNA makes exact copies itself, under the action of and control of DNA polymerase

DNA sequencing
1. The experimental process of determining the nucleotide sequence of a region of DNA. This is done by labelling each nucleotide (A, C, G or T) with either a radioactive or fluorescent marker which identifies it. There are several methods of applying this technology, each with their advantages and disadvantages. For more information, refer to a current text book. High throughput laboratories frequently use automated sequencers, which are capable of rapidly reading large numbers of templates. Sometimes, the sequences may be generated more quickly than they can be characterised
2. Procedures for determining the nucleotide sequence of a DNA fragment. There are two common methods for doing this:

the Maxam and Gilbert technique (chemical degradation), that uses different chemicals to break the DNA into fragments at specific bases; or

 

 

the Sanger technique (called the di-deoxy or chain­-terminating method) uses DNA polymerase to make new DNA chains, with di-deoxy nucleotides (chain terminators) to stop the chain randomly as it grows.

In both cases, the DNA fragments length by polyacrylamide gel sequence to be read directly

DNA topo-isomerase
An enzyme that catalyses the introduction or removal of supercoils in DNA.

DNA transformation
See transfection; transformation.

DNA delivery system
A generic term for any procedure that transports DNA into a recipient cell

Dolly
The [name of the] first mammal to be created by clothing a cell from an adult animal. In this particular case, the cell came from the mammary tissue of an adult ewe. The creation of Dolly showed that the process of differentiation into adult tissue is not, as previously thought, irreversible. The result was achieved by nuclear transfer (q.v). Dolly’s birth was announced in 1997. Since then, cattle and mice have also been cloned from adult cells.
Domain
A segment of a protein that has a discrete function or conformation. At the protein level, a domain can be as small as a few amino acid residues or as large as half of the entire protein.

Domestic animal diversity - (DAD) ill AnGR: The spectrum of genetic differences within each breed, and across

all breed within each domestic animal species, together with the species differences;
all of which are available for the sustainable intensification of food and agriculture
production.

Dominance
The type of gene action exhibited by a dominate allele (q.v.).
dominant 1. Describing an allele whose effect with respect to a particular trait is the
same in heterozygotes as in homozygotes. The opposite recessive.
2. Describing the most conspicuously abundant and characteristic species of a co
unity.
3. Describing an animal that is allowed priority in access to food, mates, etc., by
others of its species because of its success in previous aggressive encounters.

 

 

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