DNA sequencing
1. The experimental process of determining the nucleotide sequence of a region of DNA. This is done by labelling each nucleotide (A, C, G or T) with either a radioactive or fluorescent marker which identifies it. There are several methods of applying this technology, each with their advantages and disadvantages. For more information, refer to a current text book. High throughput laboratories frequently use automated sequencers, which are capable of rapidly reading large numbers of templates. Sometimes, the sequences may be generated more quickly than they can be characterised
2. Procedures for determining the nucleotide sequence of a DNA fragment. There are two common methods for doing this:
the Maxam and Gilbert technique (chemical degradation), that uses different chemicals to break the DNA into fragments at specific bases; or