Molecular Biology Dictionary,Defintion,Meaning,What is DNA Hybridization, DNA Primase, DNA micro-array, DNA polymerase

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Molecular Biology Dictionary Molecular Biology Dictionary Part 1 Part 2 Part 3 Comparative Positional Candidate Gene - Genetic Complementation Complementation / Trans Test - Conidum , Conidia Conjugation - Contig Contiguous / Contig Map - Co repressor Corpus - Coupling Covalently Closed Circle - Cross Pollination Efficiency Crown Call - Culture Room Curing - Cytoplasmic Genes Cytoplasmic Inheritance - Cytotoxic / Killer T Cell Dabs - Single Domain Antibodies - Deficiency Degeneracy of the Genetic Code - Deoxygenation Deoxyribonucleic Acid (DNA) Deoxyribonuclease (DNase) - Devitrification dG - dC Tailing - Dimer Dimorphism - Disease Disease Resistance - Diurnal Divergent Evolution / Adaptive Radiation - DNA Helicase (Gyrase) DNA Helicases DNA Hybridization - DNA Primase DNA Probe - Dominance Dominant Marker Selection - Doubler Recessive Double Stranded Complementary DNA (dscDNA) - Duplication

Home >> Molecular Biology Dictionary >> DNA Hybridization - DNA Primase

Your Ad Here	DNA hybridization The pairing of two DNA molecules, often from different sources, by hydrogen bonding between complementary nucleotides. This technique is frequently used to detect the presence of a specific nucleotide sequence in a DNA sample DNA ligase An enzyme that catalyses a reaction that links two DNA molecules via the formation of a phospho-diester bond between the 3' hydroxyl and 5' phosphate of adjacent nucleotides. It plays an important role in DNA. repair and replication. DNA ligase is one of the essential tools of recombinant DNA technology, enabling (among other thing ) the incorporation of foreign DNA into vectors. The ligase enzyme encoded by phage T4 is commonly used in gene-cloning experiments. It requires A TP as a co-factor. T4 is used in vitro to join the vector and insert DNAs .
DNA micro-array A small glass surface to which has been fixed an array of DNA fragments, each with a defined location. A typical DNA chip would contain 10 000 discrete spots (each containing a different DNA fragment) in an area of just a few square centimetres. When a solution of fluorescently labelled DNA fragments is hybridized to the chip, spots to which hybridization occurs are visible as fluorescence. If the spots on the chip are genes (expressed sequence tags, q. v.), hybridization with DNA from a particular tissue shows which genes are expressed in that tissue.
If the spots are short, synthesized oligonucleotides (approximately 25 bases) corresponding to that part of a gene containing a single nucleotide polymorphisms (SNP)(q.v.), with a separate spot for each of the 4 possible bases at that site, hybridization with genomic DNA from an individual plant or animal enables that individual to be genotyped at as many SNP loci as are represented on 'the chip. The big advantage of DNA chips is the extent to which the process of genotyping can be automated, thereby enabling huge numbers of plants or animals to be genotyped for a huge number of loci. DNA polymerase An enzyme that catalyses the synthesis of double-stranded DNA, using single-stranded DNA as a template. See polymerase.	Your Ad Here
DNA polymorphism The existence of two or more alternative forms (alleles) of a chromosomal locus that differ in nucleotide sequence or have variable numbers of repeated nucleotide units. See allele DNA primase An enzyme that catalyses the synthesis of short strands of RNA that initiate the synthesis of DNA strands.

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Molecular Biology Dictionary Part 4 Part 5 Part 6 Estimated Breeding Value - EBV - External Guide Sequence (EGS) Elastin - Embryogenesis Embryo Multiplication and Transfer (EMt) - 5 End Endogered Breed - Endomitosis Endonuclease - Enhancer Element / Sequence Enhancer - Epinasty Epiphte - Erlenmeyer Flask ES Embryonic Stem Cells - Eukaryote Euploid - Exogamy Exogenote - Exponential Phase Expresses Sequence Tag (EST) - Ex situ Preservation 3 Extension - Ex vitro Factor - Fertile Fertilizer - Fibril Fibrinogen Fibrous Protein - Fission FLAG / Affinity Tag - Foot Candle Foot Printing - Fusion Protein

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