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  Home >> Molecular Biology Dictionary >> Divergent Evolution Adaptive Radiation- DNA Helicase Gyrase

Divergent evolution
See adaptive radiation.

Dizygotic twins
Two-egg twins, i.e., a pair of individuals that shared the same uterus at the same time, but which arose from separate and independent fertilization of two ova.

DMSO
See dimethyl sulphoxide.

DNA
(deoxyribonucleic acid; formerly spelt desoxyribonucleic acid) The long chain of molecules in most cells that carries the genetic message and controls all cellular functions in most forms of life. The information-carrying genetic material that comprises the genes. DNA is a macro-molecule composed of a long chain of deoxyribonucleotides joined by phospho-diester linkages. Each deoxyribonucleotides contains a phosphate group, the five-carbon sugar 2-deoxribose, and a nitrogen-­containing base. The genetic material of most organisms and organelles so far examined is double-stranded DNA; a number of viral genomes consist of single-stranded DNA or single-or double-stranded RNA. In double- stranded DNA, the two strands run in opposite (anti-parallel) directions and are coiled round one another in a double helix. Purine bases on one strand specifically hydrogen bond with pyrimidine bases on the other strand, according to the Watson-Crick rules (A pairs with T; G pairs with C). Hence a constant width for the double helix of 20 A (2.0 nm) is maintained. In the B-form, DNA adopts a right-handed helical conformation, with each chain making a complete turn every 34 A(3.4 nm), or once every ten bases. See also mtDNA.

DNA amplification
Multiplication of a piece of DNA in a test-tube into many thousands of millions of copies. The most commonly used process is the polymerase chain reaction (PCR) system, but other systems are being developed, including ligase chain reaction (LCR), nucleic acids sequence-dependent amplification, and the Q-b system.

DNase

1/ (deoxyribonuclease) An enzyme that catalyses the cleavage of DNA. DNase I is a digestive enzyme secreted by the pancreas, that degrades DNA into shorter nucleotide fragments. Many other endonucleases and exonucleases are involved in DNA repair and replication. cf nuclease.
2. Deoxyribonuclease, a class of enzymes which digest DNA. The most common is DNase I, an endonuclease which digests both single and double-stranded DNA.

DNA bank
In AnGR: Storage of DNA, which mayor may not be the complete genome, but should always be accompanied by inventory information. (Note: at the present time, animals cannot be re-established from DNA alone.)

DNA chip
See DNA micro-array.

DNA construct
A DNA molecule inserted .into a cloning vector, usually a plasmid.

DNA diagnosis
The use of DNA polymorphisms to detect the presence of a specific allele (often associated with a disease or syndrome) or DNA sequence.

DNA fingerprint
The unique pattern of DNA fragments identified originally by Southern hybridization (using a probe that binds to a polymorphic region of DNA) or now by polymerase chain reaction (PCR) (using primers flanking the polymorphic region). See genetic fingerprinting.

DNA helicase (gyrase)
An enzyme that catalyses the unwinding of the complementary strands of a DNA double helix.

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