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  Home >> Molecular Biology Dictionary >> Conjugation - Contig

Conjugation
1. Union of sex cells (gametes) or unicellular organisms during fertilization. 2. The unidirectional transfer of DNA (bacterial plasmid) from one bacterium cell to another and involving cell-to-to-cell contact. The plasmid usually encodes the majority of the functions necessary for its own transfer.

Conjugative functions
Plasmid-based genes and their products that facilitate the transfer of a plasmid from one bacterium to another.

Consanguinity
Related by descent from a common ancestor.

Consensus sequence
1. The nucleotide sequence that is present in the majority of genetic signals or elements that perform a specific function. 2. A derived nucleotide sequence that represents a family of similar sequences. Each base in the consensus sequence corresponds to the base most frequently occuring at that position, in the real sequences. 3. A 'nominal' sequence inferred from multiple, imperfect examples. Multiple lanes of shotgun sequence can be merged to show a consensus sequence. The optimal sequence of nucleotides recognized by some factor. A DNA binding site for a protein may vary substantially, but one can infer the consensus sequence for the binding site by comparing numerous examples. For example, the (fictitious) transcription factor ZQ1 usually binds to the sequences AAAGTT, AAGGTT or AAGATT. The consensus sequence for that factor is said to be AARRTT (where R is any purine, i.e. A or G). ZQ1 may also be able to weakly bind to ACAGTT (which differs by one base from the consensus).

Conservation of farm animal genetic resources
In AnGR: Refers to all human activities, including strategies, plans, policies and actions, undertaken to ensure that the diversity of farm animal genetic resources is being maintained to contribute to food and agricultural production and productivity; now and in the future. (Source: FAO, 1999)

Constant domains
Regions of antibody chains that have the same amino acid sequence in different members of a particular class of antibody molecules.

Constitutive enzyme
An enzyme that is synthesized continually regardless of growth conditions. cf inducible enzyme; repressible enzyme.

Constitutive gene
A gene that is continually expressed in all cells of an organism.

Constitutive promoter
An unregulated promoter that allows for continual transcription of its associated gene. See promoter.

Constitutive synthesis
Continual production of RNA or protein by an organism.

Constitutive
An organism is said to be constitutive for the production of an enzyme or other protein if that protein is always produced by the cells under all physiological conditions. See inducible.

Consumers
Organisms which consume protoplasm produced from photosynthesis or consume organisms from higher levels which indirectly consume protoplasm from photosynthesis.

Contaminant
Bacterial, fungal or algal micro-organism accidentally introduced into a culture or culture medium. Contaminant may overgrow the plant cells and consequently inhibit their growth. Working under aseptic conditions with a rigorous exclusion of potential contaminants must be practised in plant tissue culture. See disinfectation; disinfestations.

Contig
1. A set of overlapping clones that provide a physical map of a portion of a chromosome. cf contiguous map. 2. A DNA sequence that overlaps with another contig. The full set of overlapping for a long region of DNA that cannot be sequenced in one run in a sequencing assay. Important in genetic mapping at the molecular level. 3. Several uses, all nouns. The term comes from a shortening of the word 'contiguous'. A 'contig' may refer to a map showing placement of a set of clones that completely, contiguously cover some segment of DNA in which you are interested. Also called the 'minimal tiling path'. More often, the term ‘contig’ is used to refer to the final product of a shotgun sequencing project. When individual lanes of sequence information are merged to infer the sequence of the large DNA piece, the product consensus sequence is called a ‘contig’.

 

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