students Logo
Home | Sitemap | Contact us | Search | Language
Left Right
  Home >> Molecular Biology Dictionary >> Clone bank, Coding sequence

Clone bank
See gene bank

Cloned strain or line
A strain or line descended directly from a clone.

Cloning

1. The mitotic division of a progenitor cell to give rise to a population of identical  daughter cells or clones.
2. Incorporation of a DNA molecular into a chromosomal site or a cloning vector.

3. Animal cloning: the creation of whole animal by mitotic divisions from single diploid somatic cell, typically by the process of nuclear transfer(q.v.).Cloning by nuclear transfer from undifferentiated embryonic cells has been possible for many years, but its widespread application has been hampered by inability to culture embryonic cells from animals other than mice. In 1997, Ian Wilmut and colleagues from Edinburgh showed that it is possible to create a whole animal from a cell taken from differentiated adult tissue, thereby opening up the possibility of widespread animal cloning. See directional cloning; megabase cloning; molecular cloning; sub-cloning; Dolly

Cloning site.
See insertion site.

Cloning vector
1.A small, self-replicating DNA molecule-usually a plasmid or viral DNA chromosome –into which foreign DNA is inserted in the process of cloning genes or other DNA sequences of interest. It can carry inserted DNA and be perpetuated in a host cell. Also called a cloning vehicle, vector, or vehicle., See vector.

2. A molecule that carries a foreign gene into a host, and allows/ facilitates the multiplication of that  gene in a host. When sequencing  a gene that has been cloned using a cloning vector (rather than by PCR), care should be taken not to include that cloning vector sequence when performing similarity searches. Plasmids, cosmids, phagemids, YACs and PACs are example types of cloning vectors.
 
Cloning vehicle
See cloning vector

Closed continuous culture
A continuous culture in which inflow of fresh medium is balanced by outflow of corresponding volumes of spent medium. Cells are separated mechanically from outflowing medium and added back to the culture. cf open continuous culture; batch culture; continuous culture.

Cluster of differentiation           
See CD.

cM
See centiMorgan; map distance

Cocoon
A protective coverage for eggs and/or larvae produced by many invertebrates, such as the silkworm moth.

Co-culture
The joint culture of two or more types of cells, such as a plant cell and a micro-organism, or two types of plant cells. Used in various dual-culture systems or in nurse-culture, q.v.

COD
See chemical oxygen demand.

Coding
The specification of a peptide sequence, by the code contained in DNA molecules.

Coding sequence
1. That portion of a gene which directly specifies the amino acid sequence of its protein product. Non-coding sequences of genes include control regions, such as promoters, operators and terminators, as well as the intron sequences of certain eukaryotic genes.
2. The portion of a gene or an mRNA which actually codes for a protein. Introns are not coding sequences; nor are the 5' or 3' untranslated regions (or the flanking regions, for that matter - they are not even transcribed into mRNA). The coding sequence in a cDNA or mature mRNA includes everything from the AUG (or ATG) initiation codon, inclusive

 

Left Right