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  Home >> Molecular Biology Dictionary >> Canola, Cat-assay

Canola
Any of several cultivars of oilseed rape (more fully: canola oil); the vegetable oil high in mono-unsaturated fatty acid obtained from these cultivars.

Cap
1.The structure found on the 5’ end of eukaryotic mRNA, and consisting of an inverted, methylated guanosine residue. See G cap.
2. All eukaryotes have at the 5’end of their messages a structure called a “cap”. Consisting of 7-methylguanosine in 5’-5’ triphosphate linkage with the first nucleotide of the mRNA. It is added post-transcriptionally, and is not encoded in the DNA

Cap site
1.The site in a gene where translation is initiated, a.k.a. translation initiation site.
2. Two usages: In eukaryotes, the cap site is the position in the gene at which transcription starts, and really should be called the “transcription initiation site”. The first nucleotide is transcribed from this site to start the nascent RNA chain. That nucleotide becomes the 5’ end of the chain, and thus the nucleotide to which the cap structure is attached (see “Cap”). In bacteria, the CAP site (note the capital letters) is a site on the DNA to which a protein factor (the Catabolite Activated Protein ) binds

Capacitation
The final stage in the maturation process of a spermatozoon, taking place inside the female genital tract as the sperm penetrates the ovum.

Capacitance
The charge-storage ability of a capacitor, defined as the magnitude of charge stored on either plate divided by the applied voltage

Capsid
The protein coat of a virus. The capsid often determines the shape of the virus. See coat protein.

Carboxypeptidases
Two enzymes (A and B) found in pancreatic juice. Their role is to remove the C-terminal amino-acid from a peptide; the A form removes any amino acid; the B form removes only lysine or arginine. Used when sequencing peptides.

Carpel
Female reproductive organ of flowering plants, consisting of stigma, style and ovary. In some plants, one or more carpels unite to form the pistil.

Carrier
In genetics, typically an individual that has one recessive mutant allele for some defective condition that is “masked” by a dominant normal allele at the same locus, i.e., an individual that is heterozygous for a recessive harmful allele and a dominant normal allele; the phenotype is normal, but the individual passes the defective (recessive ) allele to half of its offspring

Carrier DNA
DNA of undefined sequence content which is added to the transforming (plasmid)DNA used in physical DNA-transfer procedures. This additional DNA increases the efficiency of transformation in electroporation and chemically mediated DNA-delivery systems. The mechanism responsible for this effect is not known. See also binary vector; plasmid’ chimeric gene.

CAT assay
An enzyme assay. CAT stands for chloramphenicol acetyl transferase, a bacterial enzyme which inactivates chloramphenicol by acetylating it. CAT assays are often performed to test the function of a promoter. The gene coding for CAT is linked into a promoter (transcription control region) from another gene, and the construct is “ transfected” into cultured cells. The amount of CAT enzyme produced is taken to indicate the transcriptional activity of the promoter (relative to other promoters which must be tested in parallel).It is easier to perform a CAT assay than it is to do a Northern blot, so CAT assays were a common method for testing the effects of sequence changes on promoter function. Largely supplanted by the reporter gene luciferase.

 

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