Dictionary,Defintion,Meaning,What is Block Copolymer,Blut end,Blot,Blotting,Specific bands,Hybridization

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Home >> Molecular Biology Dictionary >> Block Copolymer, Blunt end

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Block Copolymer
A linear copolymer in which identical mer units are clustered in blocks along the molecular chain.

Blot
1. As a verb, this means to transfer DNA, RNA or protein to an immobilizing matrix.
2. As a noun, it usually refers to the autoradiograph produced during the Southern, or northern blotting procedures. The variations on this theme depend on the molecules:

- Southern blot: the molecules transferred are DNA molecules, and the probe (q.v.) is DNA. - northern blot: the molecules transferred are RNA, and the probe is DNA.
-western blot: the molecules transferred are protein, and the probe is labelled antibody.
-Southwestern blot: the molecules transferred are protein, and the probe is DNA.

-dot blot: DNA, RNA or protein are dotted directly onto the membrane support, so that they form discrete spots.

-colony blot: the molecules (usually DNA) are from colonies of bacteria or yeast growing on a bacteriological plate.
See DNA probes

Blotting
A technique for detecting one RNA within a mixture of RNAs (a Northern blot) or one type of DNA within a mixture of DNAs (a Southern blot). A blot can prove whether that one species of RNA or DNA is present, how much is there, and its approximate size.

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Basically, blotting involves gel electrophoresis, transfer to a blotting membrane (typically nitrocellulose or activated nylon), and incubating with a radioactive probe. Exposing the membrane to X-ray film produces darkening at a spot correlating with the position of the DNA or RNA of interest. The darker the spot, the more nucleic acid was present there. (see figure, below)
The DNA is first transferred from the gel to a membrane by capillary action. Fluid wicks from the gel through the blotting membrane to several layers of absorbent paper, but the nucleic acids stick to the membrane. Baking the filter fixes the DNA or RNA to the filter

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Specific bands are detected by hybridization. The filter membrane is incubated with radioactive probe, which hybridizes to some bands. After the filter is washed (to remove unused probe), an X-ray film exposed to the filter will show which bands have hybridized

Specific Bands are detected by Hybridization

Blunt-end
The end of a DNA duplex molecule in which neither strand extends beyond the other. a.k.a. flush end

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Molecular Biology Dictionary Part 4 Part 5 Part 6 Bioinformatics - Biosynthesis Biotic Factor - B Lymphocytes, B Cells Block Copolymer - Blunt End Blunt End Cut - Breeding Value Breed not at Risk - Burgers Vectors CAAT/CAT Box - Canine Canola - CAT Assay CAAT Box - cDNA Clone cDNA Cloning - Cell Fusion Cell Generation Time - Cell Wall Cementide - Chain Terminator Chemically Defined Medium - Chitin Chitinase - Chromosomal Aberration Chromosomal Integration Site - Chromosome Walking Cilium - Clone Clone Bank - Coding Sequence Coding Strand - Coincidence Co integrate Vector System - Comparative Gene Mapping