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  Home >> Molecular Biology Dictionary >> Autolysis - Axillary bud proliferation

Autolyis
The process of self destruction of a cell, cell .organelle, or tissue. It occurs by the action of lysosomic enzymes.

Autonomous(ly) replicating segment (or sequence)

See ARS.

Autonomous
A term applied to any biological unit that can function on its own, i.e., without the help of another unit, such as a transposable element that encodes an enzyme for its own transposition.

Autopolyploid­
A polyploid that has multiple and identical or nearly identical sets of chromosomes (genomes) all derived from the same species. A polyploid species with genomes derived from the same original species.

Autoradiograph
A picture prepared by labelling a substance such as DNA with a radioactive material such as tritiated Thymidine and allowing the image produced by decay radiation to develop on a film over a period of time.

Autosome
A chromosome that is not involved in sex determination autoradiograph

Autotraphic

1. Self-nourishing .organisms capable .of utilizing carbon dioxide or carbonates as the sole source of carbon and obtaining energy for life process from radiant energy of from the oxidation of inorganic elements, or compounds such as iron, sulphur, hydrogen, ammonium and nitrates. See heterotrophic.

2. Organisms which utilize inorganic carbon for synthesis of protoplasm. Ecologists narrow the definition further by requiring that autotrophs obtain their energy from the sun. In microbiologist parlance, this would be a photoautotroph. See photoautotrophic and chemoautotrophic.

Autotrophy
Autotrophy is the capacity of an organism to use light as the sole energy source in the synthesis of organic material from inorganic elements or compounds. Autotrophic organisms include green photosynthesizing plants and some photosynthetic bacteria. cf heterotrophy.

Autotrophs
A group of organisms capable of obtaining carbon for synthesis from inorganic carbon sources such as carbon dioxide and its dissolved species (the carbonates). This group includes plants and algae.

Auxin-cytokinin ratio

The relative proportion of auxin to cytokinin present in plant tissue-culture media. Varying the relative amounts of these two hormone groups in tissue culture formulae affects the proportional growth of shoots and roots in vitro. As the ratio is increased (increased auxin or decreases cytokinin), roots are more likely to be produced, and as it is decreased root growth declines and shoot initiation and growth are promoted. This relationships was first recognized by C.O Miller and F. Skoog in the 1950S.

Auxotroph
(Gr. auxein to increase + trophe , nourishment) A mutant cell or micro-organism lacking the capacity to from an enzyme or metabolite present in the parental strain, and that consequently will not grow on a minimal medium, but requires the addition of some compound – such as amino acid or a vitamin – for growth.

Availability

A reflection of the form and location of nutritional elements and their suitability for absorption. In tissue culture media this is related to the abundance of each nutritional element, the osmotic concentration and pH of the medium, the stability and solubility of the item in question, the presence of absorbing agents in the media, and other factors.

Axenic culture

Free of external contaminants and internal symbinots; generally not possible with surface sterilization alone, and incorrectly used to indicate aseptic culture, q.v.

Axillary bud

A bud found at the axil of a leaf (synonymous with lateral bud).

Axillary bud proliferation

Propagation in culture using protocols and media which promote axillary (lateral shoot) growth. This is a technique for mass production (micropropagation) of plantlets in culture,  achieved primarily through hormonal inhibition of apical dominance and stimulation of lateral branching.

 

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