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  Home >> Molecular Biology Dictionary >> Amplify - Animal cell immobilization

Amplify
To increases the number of copies of a DNA sequence, either in vivo by inserting into a cloning vector that replicates within a host cell, or in vitro by polymerase chain reaction (PCR).

Ampometric
See enzyme electrode.

Amylase
A group of enzymes that degrade starch, glycogen and other polysaccharides, producing a mixture of glucose and maltose. Plants have both a- and b-amylase have only a amylase.

Amylolytic

The capability of breaking down starch into sugars.

Amylose
A polysaccharide consisting of linear chains of 100 to 1000 glucose molecules. The water-soluble portion of starch.

Anabolic pathway
A pathway by which a metabolite is synthesized; a biosynthetic pathway.

Anaerobe

An organism that can grow in the absence of oxygen. Opposite: aerobe.

Anaerobes

A group of organisms that do not require molecular oxygen. These organisms, as Well as all known life forms, require oxygen. These organism ions such as nitrate or sulphate or from protein.
Analogous
Features of organisms or molecules that are superficially or functionally similar but have evolved in a different way or contain different compounds.

Anaphase (Gr. ana, up + phais, appearance)
The stage of mitosis or meiosis during which the daughter chromosomes (sister chromatids) pass from the equatorial plate to opposite poles of the cell (toward the ends of the spindle). Anaphase follows metaphase and precedes telophase

Anchor gene
A gene. that has been positioned on both the physical map and the linkage map and the linkage map of a chromosome.

Androgen
Any hormone that stimulates the development of male secondary sexual characteristics, and contributes to the control of sexual activity in vertebrate animals. Usually synthesized by the testes.

Androgenesis
Male parthenogenesis, i.e., the development of a haploid embryo from a male nucleus: The maternal nucleus is eliminated or inactivated subsequent to fertilization of the ovum, and the haploid individual (referred to as androgenetic) contains in its cells the genome of the male gamete only. Androgenesis is detected by cytological staining. See anther culture; gynogenesis; parthenogenesis.

Aneuploid

(Gr. aneu, without + ploid) An organism or cell having a chromosome number that is not  an exact multiple of the monoploid (x) with one chromosome being present in greater (e.g., trisomic 2n + 1) or lesser (e.g., monosomic 2n -1) number than the normal diploid number.

Animal cell immobilization

Entrapment of animal cells in some solid material in order to produce some natural product or genetically engineered protein. Animal cells have the advantage that they already produce many proteins of pharmacological interest, and that genetically engineered proteins are produced by them with the post-translation modifications normal to animals. However, because animal cells are much more fragile than bacterial ones, they cannot tolerate a commercial fermentation process. Typical materials are hollow fibre membrane bioreactors, or porous carries made of polysaccharide, protein, plastic or ceramic materials with microscopic holes inside which the cells grow.

 

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