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  Home >> Molecular Biology Dictionary >> Aminoacyl site;A-site - Amplification

Aminoacyl site; A-site
One of twosites on ribosomes to which the incoming tRNA binds.

Aminoacyl tRNA synthetase
Enzyme that attaches each amino acid to its specific tRNA molecule

Amitosis
Cell division (cytokinesis), including nuclear division through constriction of the nucleus, without chromosome differentiation as in mitosis. The maintenance of genetic integrity and diploidy during amitosis is uncertain. This process occurs in the endosperm of flowering plants.

Amniocentesis

A procedure for obtaining amniotic fluid from a pregnant mammal for the diagnosis of some diseases in the unborn in the foetus. Cells are cultured, arid metaphase chromosomes are examined for irregularities (e.g., Down syndrome, spina bifida, etc., in humans).

Amnion
The thin membrane that lines the fluid-filled sac in which the embryo develops in higher vertebrates, reptiles and birds.

Amniotic fluid

Liquid contents of the amniotic sac of higher vertebrates, containing cells of the embryo (not of the mother).Both fluid and cells are used for diagnosis of genetic abnormalities in the embryo or foetus

Amorph null mutation

A mutation that obliterates gene function.

Amorphous

having no long-range order.

AMP (adenosine monophosphate)

See ATP.

Amphidiploid
A species or type of plant derived from doubling the chromosomes in the F1 hybrid of two species; an allopolyploid. In an amphidiploid the two species are known, whereas in other allopolyploids they may not be known.

Amphimixis
True sexual reproduction involving the fusion of male and female gametes and the formation of a zygote.

Amplification

1. Treatment (e.g., use of chloramphenicol) designed to increase the proportion of plasmid DNA relative to that of bacterial (host) DNA.
2. Replication of a gene library in bulk.
3. Duplication of gene(s) within a chromosomal segment.
4. Creation of many copies of a segment of DNA by the polymerase chain reaction (PCR)

Amplified fragment length polymorphism (AFLP)
A type of DNA marker, generated by digestion of genomic DNA with two restriction enzymes to create many DNA fragments, ligation of specific sequences of DNA (called adaptors) to the ends of these fragments, amplification of the fragments via PCR (using a set of primers with sequences corresponding to the adapters, plus various random combinations of three additional bases at the end), and visualization of fragments via gel electrophoresis. The PCR will amplify any fragment whose sequence happens to start with any of the three-base sequences in the set of primers. AFLPs have the important advantage that many markers can be generated with relatively little effort

They are a very useful means of quantifying the extent of genetic diversity within and between populations. Their major disadvantage is that they are not specific to a particular locus and, because they are scored as the presence or absence of a band, heterozygotes cannot be distinguished from homozygotes, i.e., they are inherited in a dominant fashion.

 

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