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  Home >> Molecular Biology Dictionary >> Adsorp - Alatoxin

Adsorb
See adsorption.

Adsorbent
Noun: A substance to which compounds adhere. In tissue culture, an adsorbent is added to the culture medium to adsorb compounds released by cultured cells or minimizing any adverse effect on the subsequent growth in culture. A common adsorbent in tissue culture is activated charcoal, q.v.

Adsorption
The formation of a layer of gas, liquid or solid of a solid. cf absorption.A surface phenomena in which a solute (soluble material) concentrates or collects at a surface (the adsorbent)]

Aerobic respiration

A type of respiration in which foodstuffs are completely oxidized to carbon dioxide and water, with the release of chemical energy, in a process requiring atmospheric oxygen. aerobic Active in the presence of free oxygen.

Adult cloning
The creation of identical copies of an adult animal by nuclear transfer (q.v.) from differentiated adult tissue. See also cloning Dolly.

Advanced
Applied to an organism or a part thereof, implying considerable development from the ancestral stage or from the explant stage.
Advanced wastewater treatment

The removal of any dissolved or suspended beyond secondary treatment, often this is the :  removal of the nutrients nitrogen and/or phosphorus.

Adventitious
(L. adventitius, not properly belonging to) A structure arising at sites other than the, usual ones, e.g., shoots leaves, and embryos from any cell other than. a zygote.

Aerate
To supply with or mix with air or gas. The process is aeration.

Aerobes
Organisms which require molecular oxygen as an electron acceptor for energy production. See anaerobes.

Aerobic bacteria

Bacteria that can live in the presence of oxygen.


Affinity tag; purification tag

An amino acid sequence that has been engineered into a protein to make its purification easier. These can work in a number of ways. The tag could be another protein, which binds to some other material very tightly and thus allowing the protein to be purified by affinity chromatography (q.v.). The tag could be a short amino acid sequence, which  is recognized by an antibody. The antibody would then bind to the protein whereas it would not have done so before. One such short peptide, called FLAG, has been designed so  that it is particularly easy to make antibodies against it. The tag could be a few amino acids, which are then used as a chemical tag on the protein. For example, a string of positively charged amino acids will bind very strongly to a negatively charged filter: this could be used as the basis of a separation system. Some amino acids bind metals very strongly, especially in pairs: this chemical property can be exploited by using a filter with metal atoms chemically linked onto it to pull a. protein out of a mixture of proteins. cf affinity chromatography.

Aflatoxin

Toxic compounds, produced by moulds (fungi) of the Aspergillus flavus group, that bind to DNA and prevent replication and transcription. Aflatoxins can cause acute liver damage and cancer. Animals may be poisoned by eating stored food or feed contaminated with the mould.

 

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