Pure Culture Concept and Other Microbial Techniques, Function of Microorganisms, Micrfobial Population

Scholarships Dictionary Visa Details Study Abroad Exam Results Online Test Jobs

	Home
Microbiology Microbiology Introduction Landmarks in the History of Microbiology Discovery of Microscope Discovery of Microbial Life Abiogenesis Versus Biogenesis / Microbes and the Origin of Life Spontaneous Generation Doctrine or Abiogenesis Controversy over Spontaneous Generation End of the Debate Fermentation / A Biological Process - Pasteurization / Heating Destroys Microbes Germ Theory of Disease Koch's Postulates: The Pathogenesity Test Pure Culture Concept and Other Microbial Techniques Robert Koch 1843-1910 The Father of Microbial Techniques Growth of Medical Microbiology Causation of Diseases Bacterial Diseases and Their Discoverer Immunization Surgical Antisepsis Rise of Chemotherapy Growth of Plant Pathology Discovery of Viruses Soil Microbiology Other Important Contributions Development of Microbiology Since 1590-2000 in Chronological Form and Investors

Home >> Microbiology >>Pure Culture Concept and Other Microbial Techniques

Pure Culture Concept and other Microbial Techniques

Around 1870 it began to be realized that a sound understanding of the form and function of microorganisms could be obtained only if they are isolated and grown in pure culture form. A pure culture is one that contains only a single kind of microbial population grown from a single cell. Much of the pioneer work on pure culture technique was done by B. Brefeld (a mycologist) but his methods worked well for fungi and were found to be unsuitable for bacteria. It was Joseph Lister (1878) who first obtained pure culture of bacteria using serial dilutions in liquid media.

He took a fluid containing a mixture of bacteria, diluted it with sterile medium, and delivered it into a container of sterile milk by a specially constructed syringe. After incubation, he found that there were single kind of bacteria growing in container identical to their parent cell. In practice, serial dilution method proved to be tedious, difficult and uncertain for routine use. It also proved to be disadvantageous because one could only isolate in pure form the microbes that predominated in the original mixture. Therefore, another promising device needed to be investigated.

	Home
Scope of Microbiology Microorganisms and Principles of Biology Medical Microbiollogy and Immunology Principles of Soil Microbiology Industrial Microbiology Food Microbiology Milk and Milk Product Microbiology Water Microbiology Sewage Microbiology Air Microbiology Genetic Engineering Microorganisms in Oil Recovery Microorganisms and Energy Microorgnisms and Mining Future Prospects of Microbiology Classification of Microorganisms Basis of Their Body and Nuclear Organization On the Bassis of Organisms Classification On the Basis of Nutrition On the Basis of Oxygen Requirements On the Basis of Temperature Requirements On the Basis of Distrubution On the Basis of Economic Importance On the Basis of Osmotic Conditions Micro-Organisms in Soil / Soil Microflora Micro-Organisms in Water Microbes in Air / Air Microflora Micro-Organisms in Milk / Milk Microflora Important Microbes Present in Milk and Biochemical Changes Micro-organisms in Food / Food Microflora Common Micro-Organisms Responsible for Spoilage of Food Human Body and Microbes / Human Body Microflora