Kochs Postulates the Pathogenesity Test, Three Step Series, Pure Culture, Nutrient Media, Helath Host

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Koch's Postulates: The Pathogenesity Test

Koch first laid down in 1882 a three-step series which he said, must be fulfilled before the organism can be confirmed as the real cause of the disease. Later on, a fourth step was appended to Koch's series of conditions by E.F. Smith in 1905. These four conditions, which are now universally accepted and popularly known as "Koch's Postulates" are as follows:

1. The organism must be found associated with the disease in all the diseased hosts examined.

2. The organisms must be successfully isolated and grown in pure culture on nutrient media. Its growth characteristics be recorded.

3. The organisms should be isolated from pure culture and must be inoculated on the healthy host on which the disease appears, and it must cause the same disease on the inoculated healthy host.

4. The organism must be isolated from experimentally diseased host during the step 3 and grown in pure culture again, the organism grown in pure culture must show exactly the same characteristics of its growth which it showed during the step 2.

If all above conditions (or steps) of Koch's Postulates are proved to be true, the isolated organism is identified as the real pathogen responsible for the disease.

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