Some Examples of Improved Kinetic Parameters of Enzymes,Hydrolysis,Subtilisin Lyophilized,Acetyl Phenylalanyl Ethyl Ester

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Some Examples of Improved Kinetic Parameters of Enzymes (Modified for Acitivity in Organic Media)

Enzyme	Kcat (s-1)	KM (mM)	Kcat/KM (M-1 s-1)
Subtilisin E (ester hydrolysis)
(a) native enzyme	21 (3.3)*	0.56 (20.9)*	38 (0.16)*
(b) variant PC3	27 (62)*	0.1 (2.96)*	274 (21)*
Subtilisin lyophilized
(a) Absence of KCl	0.027	260	0.104
(b) presence of 98% KCI	10.04	26.7	390
α- Chymotrypsin lyophilized
(a) Absence of KCI	4 x 104	33	0.013
(b) Presence of KCI	220 x104	33	0.67
Subtilisin transesterification of APEE (acetyl phenylalanyl ethyl ester
(a) Salt free medium	0.0069	8.18	0.84
(b) 98% KCI medium	2.16	2.24	966

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