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Home >> Industrial and Microbial Biotechnology >> Microbes and Microbial Genomics for Industry >> Silkworm Improvement through Biotechnology

Silkworm improvement through biotechnology
Molecular maps and cloning of defense genes. Although genetic maps using classical methods have been prepared in silkworm, nothing is known about the genetics of traits influencing the yield of silk. These characters differ in multivoltine and bivoltine strains, but the genetic basis of this diversity is unknown. In view of this, at CSRTI Mysore, molecular markers have recently been used to prepare linkage maps. Linkage of these molecular markers with qualitative as well as quantitative (QTLs) traits of economic importance is also being studied in Bombax mori(silkworm).

For this purpose a cross NB I x Nistari was used (NB1 is a bivoltine, diapausing, high yielding strain, while Nistari is a multivoltine, non-diapausing, low yielding strain). DNA of B. moriwas spliced using the enzyme PstIand 200 clones (500-2000base pairs long) were raised for RFLP analysis. Efforts are also being made to develop improved strains of B. morithrough transgenesis. It is hoped that these studies at the molecular level will eventually help in bringing about significant improvement in the existing silkworm strains for high yield of silk.

Since the losses due to diseases may reach a level, as high as 30% 1or even more, considerable attention is also being paid to prevention of silkworm diseases. As a part of the effort to achieve this objective, some antibacterial and antiviral proteins have been isolated, purified and characterised. An effort is also being made to clone genes encoding these proteins, using genomic DNA and cDNA.

 

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