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Home >> Industrial and Microbial Biotechnology >> Microbes and Microbial Genomics for Industry >> Microbial Transformations

Microbial Transformations
Micro-organisms have the ability to convert one organic molecule into another. Many complex bioconversions that are achieved by micro-organisms, can not be achieved by normal chemical means and are achieved with great difficulty by using organisms other than microbes. Microbial transformations of the molecules which have found industrial application involve oxidation, reduction, isomerization, hydrolysis, condensation, etc.

Transformations of organic compounds are achieved by using the spores, growing cultures, resting cells, enzymes, immobilized cells or enzymes derived from micro-organisms. Transformation reactions at large scale are carried out under sterile conditions in aerated and stirred fermenters. Sterilization is necessary because contamination can cause production of undesired substances or can suppress the desired reaction. The end products are generally secreted outside the cell and they remain either dissolved or suspended in the fermentation broth.

For further processing, if fungus is the micro-organism used, it is separated by filtration. Recovery of the product is achieved by precipitation as calcium salt, by adsorption to ion exchangers, by extraction with appropriate solvents or by direct distillation from the medium (for volatile substances). Some of the specific microbial transformations include the following.

 

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