Immunodiagnostic kits for Diseases,Silkworm,Denosonucleosis Virus,Infectious Flacherie Virus,Nuclear Polyhedrosis Virus,Protein

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Your Ad Here	Immunodiagnostic kits for diseases. For several diseases causing great losses to silkworm, immunodiagnostic kits are also being developed at CSRTI, Mysore and NIl, new Delhi. Early detection of diseases will allow rejection of crop and replacement by healthy worms, to check spread of disease in the worm population thus avoiding economic loss to the farmers. Early detection may also allow the control of diseases in subsequent rearing. Some of the diseases of silkworm include pebrine, infectious flacherie virus (IFV), denosonucleosis virus and nuclear polyhedrosis virus (NPV).
Most of these diseases can be diagnosed by immunoassays, which are sensitive and specific. In India, with funding from DBT, CSRTI, Mysore and NIl, New Delhi have developed immunodiagnostic kits for the detection of pebrine and viral diseases. For instance, 'Protein A linked latex antisera (PALLAS)' test has been developed for the detection of pebrine in silkworms. The test can be performed within 3 minutes without the aid of a microscope. Similar tests for the detection of IFV and NPV have also been developed. These tests are being field tested and will be used at a large scale in future.
AT CSRTI, colloidal textile dye based dipstick immunoassay has also been developed as a cost effective assay because in this assay a colloidal dye is used instead of enzyme generally used in a dipstick immunoassay. The assay makes use of purified specific antibody conjugated to a colloidal textile dye reagent. The dipstick dipped in the test sample is then dipped in dye reagent for 30 minutes. A coloured dot is formed in positive samples. Upto 1000 samples can be tested in 90 minutes, in a cost effective manner.
Semisynthetic diet for silkworm Semisynthetic diet containing 25-30% mulberry has been used for silkworm in Japan and South Korea. This was done to avoid crop losses and to induce resistance in silkworms raised without mulberry leaves. A project to formulate an artificial diet was also initiated at CSRTI with the assistance of DBT. Field trials using artificial diets have already been conducted and attempts are being made to decrease the cost of diet and to improve the nutrient quality of the diet.