Gluconic Acid Production by Microbial Fermenatation,Pharmaceutical Food,Textile Leather,Photographic,Glucose Oxidation

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Home >> Industrial and Microbial Biotechnology >> Microbes and Microbial Genomics for Industry >> Gluconic Acid Production by Microbial Fermentation

Your Ad Here	Gluconic acid production Gluconic acid and its salts are widely used in pharmaceutical food, feed, detergent, textile, leather, photographic and concrete industries. During glucose oxidation, gluconic acid is produced by most Aspergillus spp. and several species of other fungal genera like Penicillium, Mucor, Fusarium, Pullularia, etc. In the industry, Aspergillus niger neems to be the most widely used micro-organism, since it contains very active form of the enzyme glucose oxidase. Glucose is the precursor for the synthesis of gluconic acid as follows:
b-D-glucose + ½ O₂ gluconic acid A typical fermentation medium for producing gluconic acid using Aspergillus sp. contains the following: glucose 150g/1; maize steep liquor 3.7g/1; KH₂P0₄ 2.0g/1; MgS0₄ 7H₂O, 0.17g/1; urea, 0.10g/1; (NH₄)₂ HPO₄, 0.4g/1: For some strains of Aspergillus niger, other nitrogen sources such as NaNO₃ NH₄N0₃ or (NH₄)₂S0₄ and peptone are also added in the medium. Maize syrup supplies the trace elements required for the fungus. The fungus is incubated in such a medium, which is kept well aerated at pH 5.5 to 6.0. Optimum yield is obtained at 28-30°C.
Often the accumulation of free gluconic acid in the medium inhibits further fungal growth, with a poor yield of the acid. To avoid this, CaC0₃ or NaOH is also added in the medium, so that gluconic acid secreted from the fungus is neutralized to Ca/Na gluconate.

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